Japanese, Thai and their F1 hybrid stocks of Pleurotus ostreatus were cultivated at 22°C during the spawn-run time, then at 15°C or 22°C during fruiting. There was no noticeable difference in change of CO2 concentrations in sawdust-ricebran substrata between Japanese and Thai stocks during spawn-run time. However, Thai stocks fruited and raised CO2 concentration of the substrata earlier than did Japanese stocks at both temperatures. At 22°C, Thai stocks formed plentiful fruit-body primordia that grew into normal fruit-bodies, while Japanese stocks formed sparse primordia that failed to follow normal growth. These traits of Japanese and Thai stocks segregated in their F1 hybrid stocks, and many F1 stocks represented the Thai phenotype.
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