The present study reports the identification of two newly recorded species of the genus Melanoleuca, M. alboflavida and M. griseobrunnea, in Japan, along with descriptions and illustration of its morphological characteristics. A slender, straight, cartilaginous, whitish stipe and lageniform to fusiform pleuro- and cheilocystidia are distinctive features of M. alboflavida. Melanoleuca griseobrunnea is characterized by a pale grey-brown pileus, a pale greyish to brownish context in stipe base, and urticiform cheilocystidia. Molecular phylogenetic analyses of the nuclear rDNA ITS region support the identity of two species, and they suggest that M. alboflavida and M. griseobrunnea are placed in subgenera Melanoleuca and Urticocystis, respectively.

We report a Hygrocybe species with dense black fibrils on a pileus and strong blackish discoloration. Based on macro- and microscopic characteristics and phylogenetic analysis, we identified this species as H. griseonigricans reported from China in 2020. This is the first report of H. griseonigricans from Japan.