We studied the effects of electrical low-frequency stimulation (ELFS) on the energy metabolism of rat hindlimb muscles by Phosphorus-31 Magnetic Resonance Spectroscopy (
31P-MRS). Energy metabolism was evaluated by the Phosphocreatine (PCr)/{Inorganic Phosphate (Pi)+Sugar Phosphate (SP)} ratio, PCr/(Pi+SP), and the intracellular pH was estimated from the chemical shift of Pi. ELFS enables skeletal muscles to produce both twitch and tetanus contractions. Twitch contraction exercise (TWE) was induced at 4Hz, and tetanus contraction exercise (TEE) at 67Hz. Stimulation voltage was 4V to produce both TWE and TEE.
The initial tension of TWE was 231.8±18.7 dynes, and that of TEE was 383.8±35.8 dynes. Later, tension of both TWE and TEE decreased with time.
The PCr/(Pi+SP) ratio in TWE was 1.2±0.1 and that in TEE was 0.6±0.1 in 2min of exercise. From 2min to the end of exercise, the ratio of both TWE and TEE returned with time. During exercise, the ratio of TWE maintained a lower value of the ratio than that of TEE. In 2min of recovery, the ratio of TWE was 3.6±0.1, and that of TEE was 2.9±0.2.
The intracellular pH of TWE was 6.84±0.03, and that of TEE was 6.62±0.04 in 2min of exercise. From 4min to the end of exercise, the intracellular pH of both TWE and TEE returned over time.
Our result showed that the PCr/(Pi+SP) ratio of both TWE and TEE returned with time during exercise, and the ratio of TWE maintained a lower level than that of TEE during exercise. To maintain a lower level of energy of TWE could stimulate synthesis of muscle protein. Therefore, TWE might be more effective to increase muscle volume or improve muscle atrophy than TEE.
The energy level of TEE returned to a greater extent than that of TWE during exercise. However, tension of both TWE and TEE did not return. This fact suggests that TEE may raise threshold intensity of excitation-contraction coupling during exercise to a great degree compared with TWE, hence, continuous TEE may increase resistance to a raised threshold intensity. Based on this fact, TEE might be more effective to improve muscle weakness and increase muscle tension than TWE.
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