Japanese Journal of Food Microbiology
Online ISSN : 1882-5982
Print ISSN : 1340-8267
ISSN-L : 1340-8267
Volume 19, Issue 3
Displaying 1-7 of 7 articles from this issue
  • Usio SIMIDU
    2002 Volume 19 Issue 3 Pages 87-94
    Published: November 30, 2002
    Released on J-STAGE: July 12, 2010
    JOURNAL FREE ACCESS
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  • 2002 Volume 19 Issue 3 Pages 95-111
    Published: November 30, 2002
    Released on J-STAGE: July 12, 2010
    JOURNAL FREE ACCESS
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  • Yotaku GYOBU, Shiho HOSOROGI, Daisuke TANAKA, Miwako SHIMIZU, Junko IS ...
    2002 Volume 19 Issue 3 Pages 113-117
    Published: November 30, 2002
    Released on J-STAGE: July 12, 2010
    JOURNAL FREE ACCESS
    Astudy of the ecology of Vibrio parahaemolyticus in the aquatic environment of Toyama Bay was carried out in late summer during the period from 1996 to 2000 . The results were as follow.1) V. parahaemolyticus counts in sea water were, in general, 0-102/100 ml at the beach, 101-3/100ml in bigger fishing ports with fish markets and 101-2/100 ml in ariver estuary. While V. parahaemolyticus counts in earth were<30/100g in beach sand, 102-3/100gin sediment of fishing ports, and 103-5/100g in mud of theriverestuary .2) V. parahaemolyticus in fishing ports appeared less frequently in surface waterthan in bottom water in near fish markets, but this pattern was reversed near the harbor entrance .3) The tdh gene was detected from all four big fishing ports examined.4) TDH-producing V. parahaemolyticus O3: K6 multiplied from 0.2/g to 1.0×108/g in sediment of fishing ports autoclaved at 25°Cfor 24 hours.
    These results show that V.parahaemolyticus in the sea water of fishing ports multiplyin sediment and mud, and that sea water from fishing ports should not be used for washing of fish, from the standpoint of food hygine.
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  • Shigezo NAITO, Daigo MATSUNAGA
    2002 Volume 19 Issue 3 Pages 119-125
    Published: November 30, 2002
    Released on J-STAGE: July 12, 2010
    JOURNAL FREE ACCESS
    Physicochemical and microbiological investigation for syneresis of Mizu-yokan (Soft bean jam) revealed the conversion of saccharide in the components by a specific bacteria, Bacillus subtilis etc.; Mizu-yokan with syneresis over 30% was accompanied by the increases of glucose, fructose, maltose and maltotriose, and the formation of levan from sucrose in the components. These phenomena were also observed in the Mizu-yokan-inoculated cells of Bacillus spp. isolated from the spoiled samples, or in jam added to culture broth from which bacterial cells had been excluded by membrane filter, suggesting that the marked syneresis of Mizu-yokan derives from the action of Bacillus spp., bacteria in the raw materials.
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  • Kazuyasu MURAKAMI, Wataru ISHIBASHI, Takaomi WADA
    2002 Volume 19 Issue 3 Pages 127-131
    Published: November 30, 2002
    Released on J-STAGE: July 12, 2010
    JOURNAL FREE ACCESS
    We tried to detect Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) strains from various samples in food preparation facilities in order to examine how MRSA contamination prevails in those fields. 1, 115 of 9, 844 samples (11.3%) were positive for S. aureus. Subsequently, 42 MRSA strains were obtained from 19 S. aureus positive samples, which mainly consisted of perishable or heat-treated dishes and compound dishes made at non-laboratory cooking facilities. The characteristics of MRSA strains were as follows: 1) coagulase type III was predominant (76.2%) in coagulase typing (I-VIII); 2) 81.0% of the strains produced neither enterotoxin (A to E) nor toxic shock syndrome toxin-1 (TSST-1); 3) 21.4%of the strai s showed strong resistance to methicillin (MIC;>200μg/ml) and most of them (88.9%) also derived from non-laboratory cooking facilities.
    In conclusion, the MRSA strains detected from samples in food sanitation fields have considerably different features from those reported in clinical materials before, whereas the chance of detection is not very frequent.
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  • Issei OKAMOTO, Kazunori ASAMOTO, Kazuto IMAI, Tadashi TERAMOTO, Minoru ...
    2002 Volume 19 Issue 3 Pages 133-137
    Published: November 30, 2002
    Released on J-STAGE: July 12, 2010
    JOURNAL FREE ACCESS
    The optimal combination of culture media for the detection of Salmonella was investigated by testing 56 samples of minced chicken meat from the market using 2 preenrichment media (BPW and EEM), 2enrichment media (RV and SBG) and 5 isolation media (BGA, DHL, MLCB, XLD and XLT4).
    1. Salmonella was detected in 46 (82-1%) of 56 minced chicken meat samples.
    2. The detection rate of Salmonella did not differ when 2-5 isolation media were used, therefore it could be increased by the use of 2 isolation media.3. The combination of BPW-RV-MLCB·XLD yielded the highest detection rate of Salmonella from minced chicken meat.
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  • 2002 Volume 19 Issue 3 Pages 140
    Published: 2002
    Released on J-STAGE: February 25, 2011
    JOURNAL FREE ACCESS
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