Japanese Journal of Food Microbiology
Online ISSN : 1882-5982
Print ISSN : 1340-8267
ISSN-L : 1340-8267
Volume 20 , Issue 2
Showing 1-6 articles out of 6 articles from the selected issue
  • Sang-Hee PARK, Kikuji ITOH
    2003 Volume 20 Issue 2 Pages 45-50
    Published: July 31, 2003
    Released: July 12, 2010
    JOURNALS FREE ACCESS
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  • Hideo IGARASHI
    2003 Volume 20 Issue 2 Pages 51-62
    Published: July 31, 2003
    Released: July 12, 2010
    JOURNALS FREE ACCESS
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  • Yasuko HARA, Maki IZUMISAWA, Kumiko ISHII, Akihisa ABE, Eiji OHASHI, T ...
    2003 Volume 20 Issue 2 Pages 63-67
    Published: July 31, 2003
    Released: July 12, 2010
    JOURNALS FREE ACCESS
    A total of 394 samples of fresh seafoods and ready-to-eat seafoods were collected from supermarkets in Tokyo, Kanagawa and Chiba for the study of Listeria spp. and Listeria monocytogenes contamination. Listeria spp. and L. monocytogenes were isolated from 10.2% and 5.8% samples, respectively. Most of the L. monocytogenes positive samples were ready-to in smoked salmon (4.3cfu/g) and boiled octopus (1.5cfu/g). The predominant serotype of L. monocytogenes was 1/2a, followed by 3a and 4b.
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  • Kayoko OTSUKA, Rie DOI, Akinobu SAITO, Keiko YANAGAWA
    2003 Volume 20 Issue 2 Pages 69-74
    Published: July 31, 2003
    Released: July 12, 2010
    JOURNALS FREE ACCESS
    We evaluated the ability of the 5'-nuclease (TaqMan) PCR assay by Commercial Detection Kit to detect Shigatoxin-producing Escherichia coli O157 in pure cultures, inoculated raw ground meat, shrimp and vegetable enrichment samples. The results were compared with the culture method, standard PCR assay and ELISA. The TaqMan PCR was able to identify the organism when 102 cfu/ml or more organisms were present in pure culture and in novobiocin-modified EC enrichment of several food samples.
    In comparison, the recovery efficiency on CTSMAC plate was from 101 to 106 cfu/ml, standard PCR assay sensitivity was 104 cfu/ml or more organisms, and ELISA test showed false positive in contaminated samples of Citrobacter freundii. Our findings indicate that TaqMan PCR assay is a useful automatic assay for identifying the existence of E. coli O157 in food samples with a high detection sensitivity and results in two days.
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  • Eiji NAGAO, Minoru NAKAGAWA, Kenji KIYOTAKI, Kenichiro KARINO, Kazue S ...
    2003 Volume 20 Issue 2 Pages 75-81
    Published: July 31, 2003
    Released: July 12, 2010
    JOURNALS FREE ACCESS
    The performance of Rapid Media-DO (RM-DO, Morinaga Milk Industry Co., Ltd.), a culture medium for rapid testing of coliforms, was experimentally examined.
    RM-DO is a prepared culture medium poured and solidified in Petri dishes in advance of testing. After a test sample is inoculated on the medium, allowed to absorb into it, and incubated, colonies that form on the surface of the medium are counted.
    RM-DO is prepared by dissolving powdered ingredients as in desoxycholate agar medium but with a greater amount of water than usual, and adjusting the water content by drying it to the prescribed level. As a result, RM-DO is able to absorb much more test sample than a conventionally-prepared plate (0.1-0.2ml), and it absorbed 1ml of a test sample within 30 minutes.
    The growth of coliforms was rapid in RM-DO, because bacteria grew on its surface, and there was no effect of heat from the poured medium, so that the incubation period could be shortened to 12 hours compared with that by the desoxycholate pour plate method (20±2 hours). In addition, the confirmatory test for coliforms could be performed simply and rapidly by directly dripping X-GAL/dimethylformamide solution over the colonies on the surface of the medium.
    In a comparative study with other media using milk inoculated with coliforms as well as raw milk, both bacterial counts and detection rate in RM-DO were comparable or superior to those by the desoxycholate agar pour plate method.
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  • Kazuaki ONO, Yoko ANDO, Rie DOI, Yukiko FUJIWARA, Yoshiko HAMADA, Kayo ...
    2003 Volume 20 Issue 2 Pages 83-86
    Published: July 31, 2003
    Released: February 25, 2011
    JOURNALS FREE ACCESS
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