Japanese Journal of Food Microbiology
Online ISSN : 1882-5982
Print ISSN : 1340-8267
ISSN-L : 1340-8267
Volume 27 , Issue 2
Showing 1-13 articles out of 13 articles from the selected issue
Symposium 2: Emerging Food-borne Infectious Diseases
Short Paper
  • Shuhei HOSOKAWA, Hidemasa KODAKA
    2010 Volume 27 Issue 2 Pages 80-85
    Published: July 31, 2010
    Released: December 13, 2010
    The Compact Dry EC (CD-EC) plate is ready-to-use test method for the enumeration of Escherichia coli and coliform bacteria in food. The plates are pre-sterilized and contained culture medium with a cold water-soluble gelling agent. The medium is re-hydrated with 1 ml of diluted sample inoculated onto the center of the self-diffusible medium, allowing the solution to diffuse by capillary action. The plate can be incubated at 35°C for 20-24hr and the colonies counted without any further working steps. The CD-EC plate method was compared with the violet red bile agar (VRBA) and MPN of the FDA-BAM methods. The correlation coefficients were 0.96 between the CD-EC plate and VRBA methods, and 0.85 between the CD-EC plate and MPN methods. Of the 142 red/pink-colony-forming isolates from the CD-EC plate method, 116 isolates were identified as Enterobacteriaceae. Blue/blue purple-colonies on the CD-EC plate method were found in 8 samples. E. coli was detected in 5 samples, however the isolates from 3 samples were identified as Escherichia vulneris. These results suggest that the CD-EC plate method is useful for screening test on the spot for E. coli and coliform bacteria in seafood.
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