Japanese Journal of Food Microbiology
Online ISSN : 1882-5982
Print ISSN : 1340-8267
ISSN-L : 1340-8267
Volume 30 , Issue 4
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Special Lecture
  • Maki HAYASHI, Hiroshi NAKAGAWA
    Type: Original
    2013 Volume 30 Issue 4 Pages 193-197
    Published: December 31, 2013
    Released: January 29, 2014
    BL-NV EIKEN, which is a novel norovirus detection kit based on bioluminescent enzyme immunoassay (BLEIA) was evaluated in a comparative study with a real time RT-PCR (rRT-PCR) for the 220 fecal specimens. As a result, the sensitivity was 94.1% (95/101), specificity was 98.3% (117/119) and concordance rate of the BLEIA was 96.4% (212/220), demonstrating that the BLEIA was equivalent to rRT-PCR with regard to the diagnostic accuracy. Furthermore, most of the 177 negative specimens were distributed from 0.2 COI (cutoff index) to 0.4 COI, indicating that the BLEIA has a satisfactory criterion which enables to distinguish positive and negative clearly. Since the BLEIA has high performance equivalent to rRT-PCR, a short turnaround time of 46 minutes, and a high throughput of 120 tests per hour, the assay is expected to be used in the screening for NV-infected food-handlers in the near future.
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