シクラメン野生種の無菌実生からの体細胞胚誘導を試みた.まず, シクラメン野生種18種の無菌播種を行った。無菌実生育成用の培地にはMS培地の大量成分を半分にしたものに, sucrose 30.0gl-1およびgellan gum3.0gl-1を加えたものを用いた.種子を発芽するまで15-20℃, 暗黒条件下に置き, 発芽した後は16時間日長および24℃の培養条件で無菌実生を育成した.Cyclamen balearicum, C. Creticum, C. intaminatum, およびC. repandumの4種の種子は発芽はしたものの, 植物体へと生育しなかった.順調に生育した14種 (C. africanum, C. cilicium, C. coum, C. cyprium, C. graecum, C. hederifolium var. album, C. libanoticum, C. mirabile, C. parviflorum, C. persicum, C. pseudibericum, C. purpurascens, C. rohlfsianum, およびC. trochopteranthum) の無菌実生組織を用いて体細胞胚誘導を試みた.無菌実生の葉柄および葉身の切片を2種類のカルス誘導培地に置床した.カルス誘導は24℃, 暗黒条件下で行った.C. cyprium, C. libanoticum, およびC. parviflorumの外植体は褐色枯死した.カルスは供試した14種のうち, 11種から得られた.カルスの形態やカルス形成率に種により差異がみられた.形成されたカルスは, 一度継代し, 増殖を促した後にカルス誘導培地からホルモンを除いた体細胞胚誘導培地に移植した.体細胞胚形成率や体細胞胚を形成した処理区において種による違いが認められた.結果としてC. africanum, C. cilicium, C. persicum, C. rohlfsianum, およびC. trochopteranthumの5種が体細胞胚を形成した.また, C. aficanumおよびC. hederifolium var. albumにおいてはシュート形成が認められた.

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Changes in the evapotranspiration (ETP) of stock (Matthiola incana R. Br.) cv. White Dorse seedlings in a cell flat were measured, and regression analyses were conducted to investigate the relationships between ETP and environmental factors (solar radiation ; SR, saturation deficit ; SD and air temperature ; AT).
In each sowing month, ETP was positively correlated with SR, SD and AT. Coefficients of determination (R²) were higher than 0.81 in SR throughout the year and larger than those in SD or AT in most sowing months. Seasonal variation of R² in the regression lines was also lowest between ETP and SR compared to the other two factors. Thus, ETP was strongly affected by SR in each sowing month.
When the yearly data were pooled, the regression line between ETP (y;mg·plant^-1) and SR (x;MJ·M^-2) was expressed as follows ; y = 276.2x+24.1 (R²= 0.873). Although R² of a multiple regression equation became 0.921 when SD and AT were included as explanatory variables, there was little difference between the values of R^-2-. A simple regression line of ETP and SR could estimate ETP.
Change in ETP during seedling growth caused by the increase in the total leaf area was only 1.7 fold in comparison to changes in total dry weight and leaf area which were 10 and 7 fold, respectively. ETP at night was considered negligible in the estimation of ETP.
When the transplants of stock are grown with an automatic fertigation system for a cell flat (12ml cell volume), supplying every 5 to 6 MJ·m^-2 cumulative solar radiation may be a suitable value for fertigation frequency.

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We demonstrated the practical utility of solar radiation mediated fertigation control for transplant production in a cell flat with an automatic ebb-and-flow fertigation system. Furthermore, we investigated seasonal changes in growth and productivity of stock (Matthiola incana R. Br.) cv. White Dorse as affected by fertigation frequency controlled in response to solar radiation (5 or 10 MJ · m^-2) and scheduled time course (once or twice per day).
The shoot dry weight of transplants and fresh weight of cut flowers were higher in plants fertigated with every 5 MJ · m^-2 of solar radiation, sown between late April and late July. In plants sown during other periods, there was no significant difference in growtn and productivity among plants fertigated differently. When the plants sown in June were fertigated twice per day, the exposure was 5 MJ · m^-2 in average of cumulatlve solar radiation from fertigation to subseauent fertigation. However, the maximum cumulative value exceeded 8 MJ · m^-2. Water stress caused by the high solar radiation accompanied by high temperature and large saturation deficit may have resulted in reduced growth of the transplants and decreased quality of the cut flowers.
When the stock transplants were grown in a cell flat (220 cells of 12ml cell volume), suffcient but not excess fertigation could be obtained by supplying nutrient solution every 5 MJ · m^-2 of solar radiation with an automatic ebb-and-flow fertigation system.

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花茎可食型を示すBrassica rarp (2n=20 AA) とB. oleracea (2n=18 CC) との複二倍体系統 (2n=38 AACC) および二基三倍体系統 (2n=29 AAC) について, 種子稔性, 生育期間, 収量および形態的な形質などを調査し, 施設栽培用の花茎可食型野菜育成の可能性を検討した.複二倍体系統の種子稔性は十分とはまだ言えないものの, 比較的高い種子稔性と自家不和合性を示す系統があり, これらは一代雑種作出の親系統として利用できる.また, 複二倍体系統にAゲノム種系統を戻し交配した場合, 高い種子稔性を示し, 二基三倍体系統は複二倍体系統より利用価値が高いと考えられる.複二倍体系統と二基三倍体系統の生育期間は二倍体系統に比べ遅れる傾向が見られたが, より生育期間の短いAゲノム種系統とCゲノム種系統を交配親に用いれば, 早生および中生系統が育成できると考えられる.一部の複二倍体系統と二基三倍体系統では栄養生長の優勢が認められ, 特に全重は著しい優勢的効果を示した.しかし, 可食率は両親より低くなった.二基三倍体系統は株型が小さく, 複二倍体系統より密植に適し, 最適な栽植密度の条件下では, 栽培面積当たりの花茎収量の増加および可食率の向上が見られた.今後, 種間複二倍体系統について, 稔性, 生育期間, 収量および形態形質の選抜によって純化をはかり, これを親とした一代雑種複二倍体および二基三倍体の実用性品種の育成が期待できる.

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Differentiation of rhizome into shoots occurred in Murashige and Skoog' medium containing 0.01-0.3μM jasmonic acid (JA), 0.01 and 0.03μM metyl jasmonate (Me-JA) and 0.01-0.3μM cucurbic acid (CUC), respectively. Differentiation was especially marked at 0.03μM of JA and 0.01μM Me-JA. Formation of protocorm-like bodies (PLB) from rhizome cultures observed at all treatments except for control treatment (growth regulator free). Higher concentrations of JA, Me-JA and CUC were effective for PLB formation. The average number of PLBs was highest at 3μM Me-JA. The rate of PLB formation was 100 percent at 1 and 3μM MeJA, and 0.3-3μM CUC treatments. Higher concentrations of Me-JA and CUC inhibited branching of rhizome cultures. Branching of the rhizome was completely inhibited by 0.1-3 μM Me-JA and 1 and 3μM CUC.

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人間協調型農業用ロボットのためのセンシングシステムの開発を目的に, 複数の人間を識別するアルゴリズムを検討した.また, センシングシステムをロボットに搭載し, マニピュレータの障害物回避実験を行った.その結果, 以下の知見を得た.
1) 人間同士の距離をパラメータに含む識別アルゴリズムでは, ある程度距離が大きい場合には良好に識別できたが, 人間が隣接した場合には対応付けできないときがあった.
2) レーザ距離計を上下移動させることで, マニピュレータが通過する高さの障害物の位置および大きさが検出され, マニピュレータは物体に接触することなく回避動作を行った.
3) レーザ距離計と赤外線センサから構成されるセンシングシステムを用いることで, 人間と背景との識別ならびに人間の移動の検出が良好に行われ, その情報をマニピュレータの情報と融合することで, 人間を含む物体に対してマニピュレータの回避制御が行われた.

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