【Purpose】This study analyses the relationship between autonomic nerve activity (ANA) and physical activity during pregnancy in primiparous and multiparous women. 【Method】We measured ANA (component coefficient of variance R-R (CVRR), low-frequency/high-frequency (LF/HF), and component coefficient of variance high-frequency (CCVHF)), and physical activity (total consumption, amount of exercise, number of steps, and walking distance) in 29 primiparous and 36 multiparous women, and calculated the time and percentage of each activity (asleep or resting, minimal exercise, low-intensity, medium-intensity, high-intensity) intensities. 【Results】There was no difference in the amount of physical activity between primiparous and multiparous women. The proportion of time spent on minimal exercise while awake was approximately 90% in both groups. In primiparous women, CVRR was positively correlated with number of steps, walking distance, and low-intensity time ; CCVHF was positively correlated with amount of exercise, number of steps, walking distance, low-intensity time, and high-intensity time, and CVRR and CCVHF were negatively correlated with minimal exercise. CCVHF and low-intensity time were positively correlated in multiparous women. 【Discussion】In both groups, sedentary behaviour, associated with lifestyle-related diseases, depression, and anxiety, accounted for approximately 90% of time spent while awake Since physical activity can activate CVRR and CCVHF and reduce depression and anxiety, the amount of both physical and low-intensity activity in primiparous women and low-intensity activity in multiparous women should be increased.