Pectic polysaccharides were extracted from the starch-free cell wall preparation of cell cultures of Marchantia polymorpha. The polysaccharides were fractionated by the chromatography on DEAE-Sephadex A50 and Bio-Gel A-5m, and depolymerized by purified glycanases. In addition, pectic polysaccharides were released as five fragments from the walls by purified endo-pectate lyase. On the basis of the analyses of these fractions, the polysaccharides were characterized. Furthermore, since several hydrolases were detected in a buffer-soluble protein fraction derived from cell homogenates, the typical pectic depolymerases such as exo-polygalacturonase and β-galactosidase were purified to homogeneity, and characterized in some detail.

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