It was investigated in the present study on what influences would be exerted on the total activity level of lactic dehydrogenase (abbr. as LDH) in porcine serum and the level of its isozyme by various factors such as the method for handling the test samples, along with evaluation of physiological values of fattening pigs. The following results were obtained.
1) Comparison of LDH activity level and its isozyme level in (heparinized) plasma and serum:
There were no significant differences between the levels of LDH activity and isozyme in (heparinized) plasma and serum.
2) Effect of leaving time from collecting blood till centrifugation upon the results of assay:
No appreciable effect was identified by the variation in the leaving time till 2 hours after collecting blood except for LDH
2 fraction.
3) Stability of LDH activity and its isozyme in serum: The stabilities were the highest when stored at -80°C followed by those at room temperature and those at -20°C in this decreasing order. Storage at 4°C proved a failure in maintaining the stability for a long period of time.
4) Effect of hemolysis upon assay results:
Neither LDH
2 nor LDH
3 received any influence upon their levels by the hemolysis, while perceivable influences upon assay results were initiated upon LDH activity from (_??_), and upon LDH
1, LDH
4 and LDH
5 from (+), (_??_) and (_??_), respectively.
5) Comparison between the assay results of blood from living bodies (abbr. as living) and blood discharged by bleeding (abbr. as bleeding):
Significant differences were found out between them in terms of LDH activity and isozyme level. However, there were significant correlation between living and bleeding, and the regression equations were approved.
6) Physiological value of fattening pigs:
To demonsrate the physiological values with mean ±2SD. The activity of serum LDH was 1, 270±448 Wróblewski unit. LDH
1, LDH
2, LDH
3, LDH
4 and LDH
5 were 34.1±12.6%, 21.2±5.6%, 22.1±5.0%, 11.7±5.8% and 11.1±7.4% respectively.
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