Pig embryos were transferred in seventeen recipient gilts by the laparoscopic technique or by the standard midventral surgery method, and the results were comparatively examined.
Donor gilts were given 1000IU each of pregnant mare serum gonadotropin, followed 72 hours thereafter by 500IU of human chorionic gonadotropin, and were then subjected to mating or artificial insemination on the first and the second day of the estrus. On day 6 or 7 (with the first day of estrus being day 0), blastocysts were collected by laparotomy from the uteri of the donors, which were maintained in Dulbecco's PBS (PBS) containing glucose (1mg/m
l) and fetal bovine serum (FBS) (10% v/v) at 37°C until the transfer to the recipients.
Recipient gilts used for the laparoscopic technique were fasted for 24 hours, then anesthetized and positioned in dorsal decubitus. Two stab incisions were made 1-2cm each in size to insert the laparoscope and the operating stick. The laparoscope and grasping forceps were then introduced into the peritoneal cavity and the ovary was examined to locate the corpora lutea.
In order to transfer the embryos, the top of one uterine horn was grasped with the forceps and a long (20cm) needle was inserted through the abdominal wall, so that the tip of the needle could reach the uterine lumen. The embryos were then loaded into a vinyl tube connected with a 1m
l syringe and transferred through the needle into the uterine lumen along with 0.4m
l of PBS containing glucose (1mg/m
l) and FBS (10% v/v).
With this laparoscopic technique, 165 embryos were transferred to eleven gilts. Three gilts (27%) which received 14, 14, and 15 embryos by implantation could farrow a total of 26 piglets. Among the recipient gilts that farrowed, the mean embryo survival was 60%.
With the midventral (surgical) technique, 92 embryos were transferred to six guts. Five gilts (83%) which received 14, 15, 16, 18 and 20 embryos could farrow a total of 47 piglets. The mean embryo survival was 58%.
The results demonstrate that the primary advantage of the laparoscopic technique was the safety of the procedure for embryo transfer and the absence of post-surgical adhesions of the internal organs. This technique is applicable to a swine embryo transfer program on general farms where suitable facilities for surgery are lacking.
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