The genetic variations of blood groups and biochemical polymorphisms in CLAWN miniature pigs were investigated in order to clarify the genetic similarities among Göttingen and Ohmini miniature strains from which this strain was originated. The blood samples were collected from twenty-five animals (13 males and 12 females) of this strain which was maintained in Kagoshima University. Blood types investigated comprised eight blood groups (A, E, F, G, H, K, L and O), five serum proteins (Tf, Pa, Hp, Cp and Am), five red cell enzymes (PHI, 6PGD, PGM, ADA and EsD) and two serum allotypes (PSA-I and PSA-II), 20 loci in total.
The results were as follows.
1) CLAWN strain showed polymorphic variations at A, E, F, H, L, PSA-I, Tf and Hp loci, while the other twelve loci were found monomorphic. From the comparison of the gene frequencies in CLAWN strain with those in Göttingen and Ohmini strains, it was presumed that they were much influenced by the genes in blood types of Göttingen and Ohmini strains from which CLAWN was derived. However, the gene frequencies at Hp and Tf loci specifically differed from those in Göttingen and Ohmini, and Tf and PSA-I loci were influenced by LW crossbred (Landrace × Large White) which was crossed once during production of this miniature strain.
2) The genetic variability of CLAWN strain revealed by gene homogenecity index (H. I.), expected proportion of heterozygosity per individual (H), proportion of polymorphic loci (P poly) and effective number of alleles per locus (Ne) was lower than that of any of the other three miniature strains (Göttingen, Ohmini and Pitman-Moore). This strain showed the second lowest variability among the thirteen pig populations investigated to date (CLAWN, Jinhua, Meishan, Taoyuan, Short-ear, Ohmini, Göttingen, Pitman-Moore, Landrace, Large White, Hampshire, Duroc and Hypor).
3) Using the gene frequencies at the 20 loci, the genetic distance from each other was calculated among thirteen pig populations on the basis of the two formulae of Rogers and Nei, and the dendrogram showing genetic similarities among them was prepared. It was clarified from the dendrogram that CLAWN strain belonged to Asian pig group which is completely different from Euro-American group, and was in a close distance to Göttingen strain.
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