日本食品科学工学会誌
Online ISSN : 1881-6681
Print ISSN : 1341-027X
ISSN-L : 1341-027X
RAPD法を用いた国内産精米の品種判別技術
大坪 研一藤井 剛橋野 陽一豊島 英親岡留 博司中村 澄子川崎 信二
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1997 年 44 巻 5 号 p. 386-390

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As it is obliged that the cultivars, location and year of production should be displayed on the bag of milled rice grains, a powerful technique to identify them by objective methods is indispensable. Milled rice grains produced at the several agricultural experiment stations were subjected to the RAPD method. Each DNA was extracted by the CTAB method and amplified by the PCR method using 10 mer primers. It became possible to identify the cultivars by electrophoresis of the PCR products among the dominant 10 cultivars in Japan.

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