Research papers on the practical examination of laboratory exercise, “Extraction and Separation of Photosynthetic Pigments” were reviewed. The methods of the extraction and separation of pigments, which appear in these papers and Biology IB textbooks for Japanese senior high school students, were examined by the present authors with the help of university students. Based on the present survey and examination, we recommend the most appropriate methods for senior high school student laboratories.
Generally, in high school biology experiments, catalase has been used when study ing enzymes. However, nowadays students are not familiar with catalase reaction because hydrogen peroxide solution has recently not been commonly used for the sterilization of wounds. In addition, since the catalase enzyme existing in the cells cannot be detected by the naked eye, its existence is difficult for students to under stand. I introduced washing detergents in my biology laboratory class to study en zymes. Most washing detergents contain two or three kinds of colored granules and one of them contains a protease. To build students’ interest in the experiment, they were asked to guess the colored granule containing the protease. For detect ing the protease activity, I used the plate method which was developed by Shino hara in 1975. As a result of the examination, the degree of interest in searching for the engyme incleased among the students. Furthermore, the students could understand the purpose of the experiment because the method was simple and the results were clear. Therefore, the enzyme experiment using the granule in the washing detergents is considered to be effective for learning about enzymes when the students are of different levels.