Japanese Journal of Biological Education
Online ISSN : 2434-1916
Print ISSN : 0287-119X
Volume 52, Issue 4
Displaying 1-4 of 4 articles from this issue
RESEARCH PAPER
  • N. Hotta, K. Chiba
    2012Volume 52Issue 4 Pages 152-164
    Published: 2012
    Released on J-STAGE: September 28, 2019
    JOURNAL FREE ACCESS

    The purpose of this study is to investigate whether university students in pre-service teacher programs of elementary schools have difficulty dealing with animals existing nearby. In this study, 23 female university students were asked to evaluate their beliefs and attitudes toward 17 types of animals easily found in backyards, including insects. The result suggested that the female students’ (1) sympathy towards insects, (2) hate against insects, and (3) ability to touch insects were improved after they experienced collection and observation of insects in the session.

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  • T. Katayama, H. Hayashi, K. Takai, Y. Endo
    2012Volume 52Issue 4 Pages 165-178
    Published: 2012
    Released on J-STAGE: September 28, 2019
    JOURNAL FREE ACCESS

    In the high school curriculum today the concept of“The central dogma” is not included in“Biology”. Only students who take the applied biology class have an opportunity to learn the concept in “BiologyⅡ”, where an E. coli cell-based recombinant system has been used for a student experiment. In the new curriculum coming in 2012, it is scheduled to be taught the central dogma at first or second grade in the first chapter of “Basic Biology”. In view of the importance in modern biology, it has been desired to have a new educational material which hits the spots in both features, effectiveness and easy-handling. Here we introduced that an in vitro simple gene expression kit is useful for visualization of transcription and translation processes in a test tube. Transcription was carried out using a phage RNA polymerase, and production of the mRNA was directly monitored using an RNA staining dye. Green fluorescent protein (GFP) was translated in the wheat germ cell- free protein synthesis system, and the product was detected directly by its fluorescence. Having established the kit, we have tested its value in the present “BiologyⅡ” classes, and could in fact confirm its usefulness. Since wheat seeds are foodstuff, the kit is free of bio-hazard and ethical issues. We expect that the kit will provide effective methods also in the new curriculum.

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RESEARCH NOTE
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