1. Isonicotinoylhydrazone, oxime, thiosemicarbazone, hydrazone, phenylhydrazone, semicarbazone and thiazolidine combined with penicillamine of pyridoxal phophate activated glutamic-oxaloacetic apotransaminase.
2. From the absorption spectra and pyridoxal phosphate content of the enzyme it was found that oxime itself combined with apo-glutamic-oxaloacetic transaminase.
3. At higher concentrations of the enzyme, it was found from its ultraviolet absorption spectrum, its pyridoxal phosphate content and the detection of the coenzyme by paper chromatography that the bound oxime remained nearly intact even after the enzyme reaction. At lower concentrations of the enzyme, where the reaction rate increased linearly with the amount of the enzyme, however, it was found by using NaBH₄ as the means to see the state of bound oxime that the oxime was decomposed after the enzyme reaction.

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It was presumed that vitamin E has its action field not only on the human arteriole system but also on the venule system from the observation of the influence of vitamin E on the variation of human skin temperature and skin blood volume caused by the change of the environmental temperature.

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Weanling male rats were fed a 18% casein diet with and without riboflavin. After 6 weeks of feeding, electroretinogram in both riboflavin deficient and control rats were examined. The ratio of amplitude in b wave/a wave was found to decrease in deficient rats. After the rats in the deficient group were returned to a normal diet for 4 weeks, the ratio become comparable to that of control rats.
Microscopic examination revealed that succinic acid dehydrogenase activity which located densely in the border of outer plexiform and inner granular layers and also in the layer of rods and cones decreased remarkably in deficient rats.

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Investigation was made on the chemical nature of thiamine-active compounds in the liver following oral administration of α-hydroxyalkyl-2-thiamine (DL-, D-, L-hydroxyethyl, DL-hydroxypropyl and DL-hydroxyisobutyl) to thiamine-deficient rats.
Bioautographical study with Lactobacillus fermenti revealed that the greater part of thiamine activity two hours after administration is accounted for by thiamine and it was concluded that this class of compound is readily converted to and exists as thiamine in the liver. No difference was observed between D-and L-isomer of hydroxyethylthiamine in the ease of this conversion.
Similar experiments with normal, healthy rats failed to produce definite evidence for the presence of hydroxyethylthiamine in the liver and kidney.

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By administering 120mg CoQ internally to diabetics every day, we observed its effect on the fasting blood sugar and ketone body levels as the index.
1. Of the 39 diabetic cases under experiment, the fasting blood sugar level showed decrease after administration of CoQ₇ in 14 cases or 36 per cent of the total. Of these cases, three indicated additional effect by joint administration of insulin and decrease of the required amount of insulin.
2. Of the 22 diabetic cases under experiment, the blood ketone bodies showed decrease after administration of CoQ₇ in 14 cases, or 63 per cent. This was particularly evident in the cases of relative hyperketonemia of more than 5mg/100ml. It was also noted that decrease of blood sugar was more conspicuous in these cases.

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1. Pantethine was administered to patients with diabetic neuropathy in daily doses of 30-200mg for 20-70 days to study its clinical effectiveness.
The substance was found effective in improving the patellar reflex or vibratory perception in extremities in some cases and especially effective against disturbance of vibratory perception in toe.
2. The increasing ratio of urinary excretion of pyruvate following intravenous injection of fructose was reduced in many cases by pantethine treatment. A correlation was noted between normalization of pyruvate metabolism and improvement in vibratory perception in extremities.

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In various organs of a rat and human, reduction of NAD by lactate dehydrogenase was found to take place with similar intensity in the presence of lactate and glyoxylate. This was evidenced by the zymogram and by the increase in absorbancy at 340mμ. A confirmatory evidence was provided by utilization of crystalline lactate dehydrogenase (M and H types).

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Recently, large amounts of ascorbic acid are used for the treatments of various diseases, so that the investigation on the urinary excretion of oxalic acid, which is one of the metabolites of ascorbic acid, was carried out in man following the administration of large amounts of the vitamin and the following findings were obtained.
1. No significant increase in urinary oxalic acid was observed following the ingestion of large amounts (1-2g) of ascorbic acid for a long period (90-180 days).
2. After ingestion of large amounts of ascorbic acid, it was eliminated in urine mainly in the reduced form of the vitamin and little was determined as diketogulonic acid, the precursor of oxalic acid.

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