The distribution of thiamine in rice kernels at different stages of maturity was studied by photographing thiochrome fluorescence, and the moisture content, weight per thousand kernels and thiamine content were also measured. The distribution of thiamine in mature rice was studied in detail.

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The distribution of thiamine in the domestic wheat, barley and naked barley at different stages of maturity was studied by the photographic method of thiochrome fluorescence, and moisture content, weight per thousand kernels and thiamine content were measured. Moreover, the distribution of thiamine in each mature kernel was studied in detail.

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The distribution of thiamine both in the dormant seed and in the seedling of kidney beans at different stages of germination (kept at 25°, for 14 days in the dark) was studied by the photographic method of thiochrome fluorescence and the moisture content, fresh weight per plant and thiamine content (total and free) of each stage were measured.

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In determining riboflavin in human urine the presence of non-riboflavin fluorescent substances disturb occasionally the fluorometry. They were shown to be anthranilic acid and its conjugate, glucuronide.
The conjugate was hydrolyzed into the free form which was identified as anthranilic acid by paper chromatography. The sugar produced was also identified as glucuronic acid by the same method.
For removing anthranilic acid conjugate, oxidation by permanganate and a preliminary chloroform extraction from alkaline solution after photolysis were found to be the most effective means.

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Evidence has been presented which indicates that in mice fed a synthetic diet the growth-stimulative effect of orotic acid was a little accelerated by adding L methionine to the diet. Successive injections of orotic acid in combination with L methionine induced reticulocyte crisis in rabbits.

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Twenty-four thiamine salts were synthesized using ion exchange resin and the methods for preparation were described in detail.

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A simple method has been devised to remove the pigments, yellow AB and OB, from the unsaponifiable fraction of margarine by treating the petroleum ether phase with glacial acetic acid containing a small amount of acetaldehyde. Applying this method, the vitamin A in the margarine containing no carotenoids can be determined easily and accurately. The recovery of vitamin A throughout the whole procedure was found to be above 96 per cent.

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In the simple assay method of vitamin A in margarine which has been devised by the authors, yellow AB and OB were found by condensing with acetaldehyde to form phenylazo-acetaldehyde-β naphthylimide or o-tolylazo-acetaldehyde-β-naphthylimide which can be removed easily by acetic acid.
Various phenylazo-aldehyde-β-naphthylimides and o-tolylazo-aldehyde-β-naphthylimides were prepared from yellew AB or OB and their chemical properties were described.

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1. A marked decrease in liver CoA was observed in rats maintained on VB₆-deficient diets.
2. The enzymes fractionally prepared from the liver of rats and pigs according to the method of Hoagland and Novelli were employed for the biosynthesis of CoA, and VB₆ appears to be involved in the synthesis of pantetheine from pantothenate.

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1. Urinary excretion of PaA and the increase in the excretion of the vitamin following pantothenate administration were measured in 52 patients with various liver damages. Mutual relations with various liver functions were also studied.
2. Both urinary PaA and the increase in excretion following intramuscular administration of the vitamin were reduced in most patients in comparison with those in normal adults.
In relatively severe cases, increase in PaA excretion following intramuscular administration of the vitamin was frequently found to be greater than that of normal persons.
3. Correlation was found between PaA excretion and various liver functions, especially, TTT, serum cholesterol ester ratio, hippuric acid synthesis and blood pyruvate level.
4. The results mentioned above led to the conclusion that a metabolic disturbance of PaA may exist in liver damages leading to the impairment of liver functions, especially, hippuric acid synthesis, and the metabolism of α-keto acid and cholesterol.

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1. Stool samples were cultured for 2 weeks in glucose-broth media containing 5mg/100ml riboflavin, those showing a decrease in riboflavin up to below 3mg/100ml were assumed to be positive for riboflavin-decomposing bacteria.
The incidence of positive stool was 6.3per cent, most frequent in elderly subjects, followed by children under 10 years of age. It was not found in breast-fed infants.
2. Two strains of riboflavin-decomposing bacteria, 14 I and A I, were isolated in a pure state from one stool sample showing marked riboflavin decomposition.
3. The 14-I strain is a motile, non-spore-forming, Gram-positive rod which alone markedly decomposes riboflavin.
4. The A-I strain, too, is a motile, non-spore-forming, Gram-positive rod but differs in toto from the 14-I strain both bacteriologically and immunologically.
The riboflavin-decomposing activity of this bacterium alone is weak but when cultured together with the A-IV strain, isolated from the same stool sample, a marked decomposition of riboflavin takes place.
The A-IV strain is a motile, non-spore-forming, Gram-positive rod, which alone does not break down riboflavin.
5. The break down product of all these riboflavin-decomposing bacteria is lumichrome. Riboflavin decomposition is accelerated in shaking culture, but no decomposition occurs when glucose is added to the medium.

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