All issues
Volume 36, Issue 8
Displaying 1-14 of 14 articles from this issue
- |<
- <
- 1
- >
- >|
-
KOSAKU NITTA, NOBUO HORIBA, KEIKO UCHIDA, TAKAAKI TSUTSUI, SHIGERU HOR ...1994 Volume 36 Issue 8 Pages 883-889
Published: 1994
Released on J-STAGE: July 05, 2010
JOURNAL FREE ACCESSBovine subcultures (second passage) of glomerular endothelial cells (GEN) isolated from oneyear-old kidney were successfully transfected by recombinant plasmids containing the simian virus (SV)-40 T antigen (Tag) using a lipofectin-mediated procedure. One cell clone was selected, propagated and characterized. This clone can be grown in RPMI 1640 medium supplemented with 10% fetal calf serum. The advantage of this cell line is the cultivation of bovine GEN without the addition of fibroblast growth factor or a coating of fibronectin or gelatin on the culture plate. More than 80 passages were achieved and the doubling time was 32 h. The Tag was easily identified in transfected-GEN by indirect immunofluorescence. These cells weakly expressed factor VIII-related antigen, slightly took up acetylated-low density lipoprotein and secreted a detectable amount of angiotensin-converting enzyme. Immunocytochemical staining for UAE-1 was also positive. Moreover, oncoproteins, such as Ki-67 and p53, were expressed in these cells. Cell cycle analysis by flow cytometry revealed that the percentages of G1, S, and G2/M stages in cycling transfected-GEN culture in RPMI 1640 medium supplemented with 10% fetal calf serum were 34%, 52.9%, and 13.1%, respectively. The conditioned medium from confluent transfected-GEN stimulated ['H]thymidine incorporfition into glomerular mesangial cells. This cell line may provide a useful tool for examining modulators of mesangial cell growth. Thus this cell line is the first immortalized bovine GEN that retain the morphologic, phenotypic, and functional characteristics of bovine GEN.View full abstractDownload PDF (2762K) -
HIDEO SHIIKI, YASUHIRO ENOMOTO, HIDETO UYAMA, TOSHIHIKO NISHINO, YASUH ...1994 Volume 36 Issue 8 Pages 890-895
Published: 1994
Released on J-STAGE: March 01, 2011
JOURNAL FREE ACCESSThrombomodulin (TM), an endothelial cell surface glycoprotein, is a regulatory factor in the intravascular anticoagulant system. Furthermore, its plasma level is believed to reflect injury to the endothelial cell. In searching for changes in intraglomerular coagulation and endothelial cell injury during the clinical course of 14 patients with focal and segmental glomerulosclerosis (FSGS), we cvaluated the distribution of thrombomodulin (TM) in the kidney by immunohistochemical methods. In the nephrotic stage, intraglomerular staining for TM was weak and segmental and occurred in 6 out of 9 patients (67%), but the incidence of TM expression was not different significantly from that in the normal kidney. Sclerotic lesion was negative for TM. In all patients with incomplete remission and with complete remission, strong and diffuse staining was seen in intra- and extraglomerular endothelial cells. Moreover, TM was scattered in sclerotic lesions. The present study suggest that the over-expression of TM in remission may be linked to recovery from endothelial cell damage and that TM may be closely involved in the repair of FSGS.View full abstractDownload PDF (3550K) -
TOSHIAKI SHIBASAKI, TAEKO MISAWA, HIROSHI MATSUMOTO, SEIJIRO ABE, HIRO ...1994 Volume 36 Issue 8 Pages 896-901
Published: 1994
Released on J-STAGE: July 05, 2010
JOURNAL FREE ACCESSPrimary nephrotic syndrome can, although infrequently, cause severe anemia. However, the mechanisms of the anemia remain unknown. We investigated the mechanism of anemia in nephrotic syndrome by measuring parameters of nephrotic syndrome and anemia in 44 nephrotic patients (male: female; 21:23, average age; 43.6 ± 20.3 years). Nephrotic patients had significantly lower hematocrits than did healthy controls (43.3 ± 3.7 vs. 46.8 ± 3.4% in males, 37.4 ± 3.5 vs. 40.8 ± 2.8% in females). Serum crythropoietin (Epo) concentrations were correlated inversely with hemoglobin (Hb), hematocrit (Hct), and red blood cell corpuscle (RBC) counts. Furthermore, serum Epo correlated with the scrum iron concentration, but not with the other parameters, such as reticulocytes, scrum protein and proteinuria. However, the maximum Epo concentration was less than 100 mU/ml in spite of severe anemia, and this was thought to be inappropriate. On the contrary, urine Epo was not detected by the same method of serum Epo determination in spite of aggressive dialysis with distilled water. When four patients with severe anemia were subcutaneously administered recombinant Epo 6, 000 unit two times a week, they showed marked improvement in Hb/Hct/RBC. The precise mechanism of anemia in NS was not elucidated by this investigation, but further study should clarify the causes of the inappropriately low concentration of scrum Epo in patients with primary nephrotic syndrome.View full abstractDownload PDF (705K) -
EISEI NOIRI, TOSHIHIRO OKUDA, TOMOKO TAKANO, KIYOSHI KUROKAWA1994 Volume 36 Issue 8 Pages 902-906
Published: 1994
Released on J-STAGE: July 05, 2010
JOURNAL FREE ACCESSIn the present paper, we report four very elderly patients (80 years of age or older) with primary nephrotic syndrome. In all patients, oral prednisolone (PSL) alternative day therapy was attempted and three patients responded very well (complete remission 2, partial remission 1). However, one patient exhibited no effect of PSL and died from acute oliguric renal failure in spite of aggressive measures including hemodialysis. Fracture of the femoral neck occurred in one patient during PSL therapy, although the relationship between the fracture and PSL therapy was uncertain. In addition to our reported cases, 5 cases of nephrotic syndrome in similar patients reported elsewhere arc analyzed together with our cohort. PSL was commonly effective in inducing remission in very elderly patients (7 of 9 patients: 78%). Very elderly patients with nephrotic syndrome frequently suffer from oliguric renal failure, which has a potentially high mortality. Based on these data, we conclude that PSL therapy can ameliorate primary nephrotic syndrome in patients over the age of eighty years. However, the complications both of the disease and its treatment, (e.g., acute oliguric renal failure, hip fracture) must be carefully monitored.View full abstractDownload PDF (661K) -
YUJI MORIMOTO, KANAME OKADA, SHOJI KAGAMI1994 Volume 36 Issue 8 Pages 907-913
Published: 1994
Released on J-STAGE: July 05, 2010
JOURNAL FREE ACCESSAssessment of activity of lupus nephritis is critical in the management and ultimate prognosis of patients. Clinical and laboratory data of 18 children with lupus nephritis were examined to determine what factors reflect activity of renal lesions. Using a histologic scoring system with an activity index (AI) and a chronicity index (CI), correlations between histologic secores and laboratory parameters were analyzed. Serum total protein, albumin and complement at the time of renal biopsy inversely correlated with the AT, but no parameters correlated with the CI. Serum complement levels correlated inversely with the amount of glomerular immune deposits, while the AT and CI correlated with the amount of immune deposits. These results suggest that hypoalbuminemia and hypocomplementemia are indicators of severe, active lupus nephritis, pointing to the need for early renal biopsy and intensive therapy.View full abstractDownload PDF (757K) -
TERUYO KUSABA, KOICHI YAMAGUCHI, HARUKO ODA, TAKASHI HARADA1994 Volume 36 Issue 8 Pages 914-920
Published: 1994
Released on J-STAGE: July 05, 2010
JOURNAL FREE ACCESSTwenty-eight chronic hemodialysis patients were studied, and 118 consecutive measurements of the diameter of their inferior vena cava (VCD) were performed with ultrasonography. There was a significant correlation between the percent change in VCD in the expiratory phase (ΔVCD-E%) and the percent change of in body weight (ΔBW%o). The average VCD-E in predialysis was 10.7 ± 3.2 mm/m2 and this value decreased gradually following ultrafiltration and reached a minimal diameter of 7.5 ± 2.8 mm/m2 in postdialysis. Three cases developed hypotension due to fluid removal. Their VCD-E value showed a rapid reduction below 7.5 mm/m2 in the early phase of hemodialysis, then maintained a plateau. The hypotension was thought to be caused by hypovolemia due to overdehydration. We conclude that VCD-E was an effective indicator for determining the volume of fluid removed, and the dry weight was attained safely and correctly by means of undergoing ultrafiltration to keep the value of VCD-E above 7.5 ± 2.8 mm /m2.View full abstractDownload PDF (4185K) -
MITSURU KUBO, EIKI MURAKAMI, KEISUKE MATSUZAKI, MIKIO MUKAI, YUKIO KAZ ...1994 Volume 36 Issue 8 Pages 921-927
Published: 1994
Released on J-STAGE: July 05, 2010
JOURNAL FREE ACCESSThe main cause of death in chronic hemodialysis patients is heart failure. We studied cardiac function prospectively in diabetic and non-diabetic patients who had recently been started on hemodialysis. We analyzed the mechanocardiograms and echocardiograms in addition to routine blood chemistry tests, chest X-rays, and electrocardiograms of ten hemodialysis patients at our hospital from 1989 to 1991. Ejection fraction (EF) and fractional shortening (FS) were not significantly different between diabetic patients and non-diabetic patients at the start of hemodialysis. After starting hemodialysis, EF and FS declined in diabetic patients. In contrast, non-diabetic patients did not show any changes in EF and FS during hemodialysis. The results demonstrated a poor prognosis in cardiac systolic function in hemodialysis patients with diabetes mellitus.View full abstractDownload PDF (751K) -
MASAO KANAUCHI, KENJI ISHII, KIMIAKI NISHIURA, KAZUHIRO DOHI1994 Volume 36 Issue 8 Pages 928-933
Published: 1994
Released on J-STAGE: March 01, 2011
JOURNAL FREE ACCESSWe investigated the effects of exercise on hemodynamics and urinary protein excretion in 15 patients with early-stage diabetic nephropathy (DN) as compared the findings with these of 16 healthy volunteers. Patients were divided into two groups according to their renal histopathologic findings: Group DO consisted of 8 patients in whom light microscopy showed minor glomerular abnormalities and Group DI consisted of 7 patients with early stage diffuse lesions. The subjects exercised on a treadmill at a workload of 4.7 METS for 20 minutes. Systolic blood pressure, heart rate and the pressure rate product were significantly higher in the DI group than in the DO and control groups during exercise. Diastolic blood pressure was similar among the three groups. Creatinine clearance was unchanged during exercise. Urinary albumin excretion, urinary acid soluble protein excretion and urinary α1-microglobulin excretion were all significantly increased in group DI compared with the DO and control groups. Excretion of β2-microglobulin and N-acetyl-β-D-glucosaminidase activity were unchanged during exercise. Our findings suggest that this provocative exercise test is useful for diagnosing early stage diabetic nephropathy.View full abstractDownload PDF (638K) -
TARO MINAGAWA, TAKAHIRO HIRANO, NOBUYUKI TAKADA, MOTOVUKI ISHIGURO, HI ...1994 Volume 36 Issue 8 Pages 934-941
Published: 1994
Released on J-STAGE: March 01, 2011
JOURNAL FREE ACCESSThe purpose of this study was to examine the effect of recombinant human erythropoietin (r-HuEPO) on left ventricular mass. Twenty-seven hemodialysis patients (13 men and 14 women) were given r-HuEPO for renal anemia. Blood pressure and heart rate were measured before and after the 16-week course of r-HuEPO, and at the same time echocardiography was performed to measure left ventricular dimensions and wall thickness. These measurements were used to calculate left ventricular volume, cardiac output (CO), and left ventricular mass (LVmass). Diastolic blood pressure (DBP) increased after administration of r-HuEPO (from 75.8 ± 10.5 mmHg to 85.6 ± 12.7 mmHg), but there was no change in systolic blood pressure (SBP) or heart rate. LVmass increased significantly in seven cases (from 194.7 ± 40.O g to 240.3 ± 47.3g). These cases, Group I, showed no decline in stroke volume (SV) or CO, and showed significant increases in SBP. In the remaining 20 cases, Group II, LVmass decreased or was unchanged. In this group SV and CO decreased, but there was no increase in SBP. We conclude that increases in LVmass may be associated with elevated systolic blood pressure and hypertrophy of the left ventricular wall, when hemodialysis patients with severe renal anemia are given r-HuEPO.View full abstractDownload PDF (838K) -
TOSHIMASA HISHIKI, YASUHIKO TOMINO, SACHIKO INOKUCHI, ISAO SHIRATO, MI ...1994 Volume 36 Issue 8 Pages 942-946
Published: 1994
Released on J-STAGE: July 05, 2010
JOURNAL FREE ACCESSTwo adult patients with mesangial proliferative glomerulonephritis with diffuse IgM deposition in the glomcruli are reported. Case 1 was a 25-year-old female with nephrotic syndrome who showed complete remission after treatment with prednisolone (PSL). Case 2 was a 46-year-old male with asymp tomatic proteinuria who showed incomplete remission (0.5-1.0 g/24 hr) of urinary protein without any medication. In light microscopy, these patients revealed minimal or slight proliferation of glomerular mesangial cells without glomerular sclerosis and crescent formation. Deposition of IgM and C3 was observed in the glomerular mesangial areas and capillary walls by immunofluorescence. Electron-dense deposits were observed in the glomerular mesangial areas in these patients. Mesangial proliferative glomerulonephritis associated with diffuse IgM deposition in the glomcruli appears to have a benign clinical course. It has also been suggested that this disease has variant clinical courses since we recently experienced two other patients with mesangial proliferative glomerulonephritis with focal IgM deposits who showed renal tubular dysfunction or chronic renal failure.View full abstractDownload PDF (4894K) -
TETSUO SHIBATA, EIJI OKABE, AKIHIRO SUMIE, TAKANORI ISHII, TADASHI TOM ...1994 Volume 36 Issue 8 Pages 947-950
Published: 1994
Released on J-STAGE: July 05, 2010
JOURNAL FREE ACCESSA 19-year-old male developed nephrotic syndrome during the course of chronic ostcomyelitis complicating a traumatic suppurative arthritis of the knee. Renal biopsy revealed severe mesangial proliferative glomerulonephritis, and immunofluorescent microscopy demonstrated the presence of IgA. Nephrotic syndrome remitted during the treatment for chronic osteomyelitis, suggesting a close association of the two conditions.View full abstractDownload PDF (5209K) -
TOHRU KOKUBO, YOSHIYUKI HIKI, AKIRA HORII, YUTAKA KOBAYASHI1994 Volume 36 Issue 8 Pages 951-954
Published: 1994
Released on J-STAGE: July 05, 2010
JOURNAL FREE ACCESSA 68-year-old woman who had a history of Recklinghausen neurofibromatosis from 1964 showed nephrotic syndrome in 1989. Renal biopsy revealed membranous nephropathy. Various suspected causes of secondary membranous nephropathy were not found. Coexistence of Recklinghausen neurofibromatosis and membranous nephropathy has rarely been reported.View full abstractDownload PDF (2985K) -
SHINICHIRO KAWAI, MASHAHIKO YAMADA, GENGO OSAWA1994 Volume 36 Issue 8 Pages 955-961
Published: 1994
Released on J-STAGE: July 05, 2010
JOURNAL FREE ACCESSA 20-year-old man with hypouricemia with markedly increased renal uric acid clearance is described. He also exhibited idiopathic hypercalciuria and lipoid nephrosis on hospital admission. Urate excretion was minimally suppressed by pyrazinamide and minimally increased after administration of probenecid, whereas it decreased after administration of benzbromarone. These results suggest that not only presecretory reabsorption, but also postsecretory reabsorption of urate was incompletely defective and indicate that the latter is more defective than the former. The relationship between hypouricemia and hypercalciuria or lipoid nephrosis in this case is not clear. However, since the hypouricemia persisted despite the remission of minimal change nephrotic syndrome, it appears that at least his nephrosis was incidental.View full abstractDownload PDF (815K) -
HIROKAZU TAMURA, HITOSHI KATO, SATORU HIROSE, SHINJI ITOYAMA, OSAMU MA ...1994 Volume 36 Issue 8 Pages 962-967
Published: 1994
Released on J-STAGE: July 05, 2010
JOURNAL FREE ACCESSCongenital hepatic fibrosis is often associated with infantile, but not with adult polycystic kidney disease. We report the unusual case of an adult patient with polycystic kidney disease complicated by congenital hepatic fibrosis. A 27-year-old women was admitted to our hospital because of gross hematuria due to hemorrhage from renal cysts. She presented hematemesis from ruptured esophageal varices at the age of 14 years. She was diagnosed as having end-stage renal disease due to polycystic kidney disease at the age of 23 years, and maintenance hemodialysis was initiated the following year. Gross hematuria was managed with supportive therapy. However, the patient developed cholangitis and died of sepsis. Postmortem examinations as well as the patient's clinical course suggested that she had an autosomal dominant type of polycystic kidney disease. Histological findings of the liver were compatible with congenital hepatic fibrosis.View full abstractDownload PDF (7775K)
- |<
- <
- 1
- >
- >|