Japanese pear (
Pyrus pyrifolia) exhibits gametophytic self-incompatibility (GSI) controlled by a complex and multiallelic
S locus. The pistil-part product of the
S locus is the polymorphic ribonuclease, S-RNase. Information on
S-genotypes is important for the production and breeding of Japanese pears. Molecular analyses of
S-genotypes of Japanese pear have been conducted with the CAPS (cleaved amplified polymorphic sequence) system; PCR amplification of
S-RNase fragments by a common primer pair followed by digestion with restriction enzymes each of which cleaves a specific
S haplotype. Here, we show that the separation of
S-RNase fragments by polyacrylamide gel electrophoresis (PAGE) distinguishes four out of nine
S haplotypes of Japanese pear without restriction digestion.
S3-,
S5-,
S6- and
S8-
RNases were identified as distinct bands by PAGE.
S3- and
S5-
RNases were separated by PAGE despite their identical fragment sizes. Using this system, three Japanese pear lines with unknown
S-genotypes were analyzed. The newly determined
S-genotypes of the lines were confirmed by CAPS analysis.
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