The dried product of sea-weed Undaria pinnatifida (dried ‘Wakame’ product) which has a deep black color has been judged to be of high quality. In this paper, the relations between the quality and chlorophyll contents in dried ‘Wakame’ products were investigated. The following results were obtained.
1. The absorption maxima of the pigments extracted from good quality specimens with 80% acetone shifted into the range of 660.5-662nm for the “red” maxima and 425-427nm for the “blue” maxima, giving the blue/red absorbance ratio of 1.73-1.94. In inferior quality specimens the absorption maxima were 666-667nm and 406-409.5nm with the blue/red absorbance ratio of 2.65-2.97.
Thus, it was evident that the spectrophotometric properties of pigments extracted from good quality specimens were similar to chlorophyll a, although the absorbance ratio was larger than for chlorophyll a (1.24-1.28); pigments of inferior specimens were similar to pheophytin a, although the absorbance ratio was larger than for pheophytin a (2.03-2.08).
2. Larger amounts of chlorophyll a were contained in good quality specimens (107.9-268.5mg/100g) compared with the amounts in inferior specimens (0-34.2 mg/100g); the contents of pheophytin a (0-70.2mg/100g) in good specimens were smaller than in the inferior specimens (154.4-645.1mg/100g).

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We used silica gel TLC of the pigments of dried ‘Wakame’ (Undaria pinnatifida) to confirm the existence of some altered chlorophyll a and chlorophyll c which stayed on the baseline.
In the pigments extracted from dried ‘Wakarne’ of high quality several bluegreen spots appeared close to Rf 0.4 on silica gel plate when developed by n-hexan-diethyl ether-acetone (6:3:2, v/v). These pigments were considered to be degradation products of chlorophyll a.
When the chlorophyll a fraction separated by sucrose column chromatography from whole pigments of fresh ‘Wakame’ was stored in a dark room for 2 weeks at 5°C, it produced two clear blue green spots with some faints green spots. But pigments of ashed ‘Wakarne’ (Haibashi-wakame) showed two blue-green spots, one of which shifted to Rf 0.40, and also others which were chromatographically similar to chlorophyll b of spinach pigments and shifted to a lower position on the silica gel plate.
The blue and red absorption maxima of A₁, B₃, and C₂ pigments were in the range of 427-430nm and 661-66.2nm in diethyl ether. The blue/red absorbance ratios in pigments A₁ and C₁ (l.45 and 1.35) were larger than in B₃ (1.30) and common chlorophyll a (1.29-1.30). These pigments were confirmed to be phytol; their IR spectra absorptions appeared near 1740, 1700, and 1650 cm^-1 in CCl₄. From these results it was deduced that A₁, B₃, and C₁ pigments were similar to the chlorophyll a contained in spinach and other plants.
The pigments B₁ and B₂ did not contain phytol when hydrolysed. It seems that the pigment B₁ was the Mg-free derivative because the absorption near 1650 crn^-1 in the IR spectra in CCl₄ was not present.
The absorption spectra of pigment B₄ differed from pigments B₁-B₃ considerably, shifting to 410nm on the blue and 655nm on the red and having a blue/red absorbance ratio of 3.15. The IR spectra of pigment B₄ was similar to C₂ in CCl₄; a prominent absorption near 1740cm^-1 and a weak absorption near 1650cm^-1 were observed, but the one near 1710cm^-1 was not seen. The absorption maxima (666, 410nm) of pigment B₅ appeared as a grey spot on the silica gel plate (Rf 0.58). This result was similar to that for pheophytin a (666, 409nm), although the blue/red absorbance ratio (2.14) was higher than for pheophytin a (2.03-2.08).

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