A sensitive solid-phase spectrophotometric method for the determination of phenol in tap water or river water samples was developed. The antipyrine dye formed by the reaction between phenol and 4-aminoantipyrine was extracted and concentrated from 50 cm
3 of sample solutions on the finely divided anion-exchange resin in the presence of sodium sulfate (14.5 g/50 cm
3 of a sample solution) as the salting out reagent. After the resin was collected on a membrane filter by filtration under suction as a colored circular thin layer (
ca. 17 mm in diameter), the absorbance of the antipyrine dye fixed on the resin thin layer was measured directly on the membrane filter. Calibration graphs were prepared in the range 0 - 1.0 μg of phenol. The calibration graphs consist of two linear regions, 0 - 0.6 μg and 0.6 - 1.0 μg. The relative standard deviations were 1.9 and 0.2% for five replicate determinations of a blank and 1.0 μg of phenol, respectively. The detection limit, based on three times the standard deviation of the blank, was 25 ng of phenol for 50 cm
3 of solution (0.5 ng cm
-3). The recovery test was done by adding a 0.2 or 0.4 μg amount of phenol to 50 cm
3 of tap water or river water samples and determining phenol by the proposed method with or without separation by distillation of phenol. The recoveries were 90 - 110% and the relative standard deviations were 1.8 - 15% for the tap water samples analyzed with or without distillation and the river water sample with distillation. On the other hand, recoveries were 110 - 120% for the river water sample analyzed without distillation.
抄録全体を表示