This article aims to overview infrared (IR) spectroscopy. Simultaneously, it outlines mid-infrared (MIR), near-infrared (NIR), and far-infrared (FIR) or terahertz (THz) spectroscopy separately, and compares them in terms of principles, characteristics, advantages, and applications. MIR spectroscopy is the central spectroscopic technique in the IR region, and is mainly concerned with the fundamentals of molecular vibrations. NIR spectroscopy incorporates both electronic and vibrational spectroscopy; however, in this review, I have chiefly discussed vibrational NIR spectroscopy, where bands due to overtones and combination modes appear. FIR or THz spectroscopy contains both vibrational and rotational spectroscopy. However, only vibrational FIR or THz spectroscopy has been discussed in this review. These three spectroscopy cover wide areas in their applications, making it rather difficult to describe these various topics simultaneously. Hence, I have selected three key topics: hydrogen bond studies, applications of quantum chemical calculations, and imaging. The perspective of the three spectroscopy has been discussed in the last section.

Phthalate esters are synthetic chemicals that are widely used in plastic industries as plasticizer. They are harmful to humans and could be carcinogenic. In this research, a new nanosorbent was prepared via a Schiff-base reaction between p-dimethylaminobenzaldehyde and Fe₃O₄@SiO₂-NH₂ nanoparticles. A characterization of the sorbent was performed by Fourier-transform infrared spectroscopy, transmission electron microscopy, scanning electron microscopy, and energy-dispersive spectroscopy. A modified nanosorbent has a core shell structure, and shows a great tendency towards the sorption of phthalate esters. Hence, it was utilized for the dispersive solid-phase extraction of six phthalate esters and determination by gas chromatography–flame ionization detection. Several variables, such as the pH, sorbent amount, salt effects, extraction and desorption time, extraction solvent type and volume, were investigated to establish the optimal conditions. Calibration graphs were linear in the range of 1.0 – 150.0 μg L⁻¹ for dimethyl phthalate, bis-(2-ethylhexyl) phthalate, di-n-octyl phthalate and 0.1 – 200.0 μg L⁻¹ for diethyl phthalate, di-n-butyl phthalate and butyl benzyl phthalate, respectively. The obtained limits of detections (S/N = 3) were in the range of 0.02 – 0.31 μg L⁻¹. Application of the method for the enrichment and determination of phthalate esters in mineral water, natural low fat yogurt and sodium chloride infusion (0.9%, w/v) was investigated.

A convenient homogeneous electrochemical thrombin sensor based on potential-assisted Au–S deposition and a dual signal amplification strategy was established in this study. Potential-assisted Au–S deposition does not require the modification of the gold electrode, thus eliminating the tedious pre-modification of the electrode. To better amplify the output signal, both ends of the signal hairpin probes were modified with a new electroactive substance, tetraferrocene, which was synthesized by the authors. Thrombin was immediately hybridized with a thiol-modified probe to open the stem-loop structure. After chain hybridization, thrombin was replaced and participated in the next round of the reaction; thus, the cascade amplification of the signal was realized. The hybrid chain formed an Au–S deposition under potential assistance, and the electrochemical signal of tetraferrocene could then be measured through differential pulse voltammetry (DPV) and consequently used for the quantitative detection of target thrombin. In addition, the detection limit of thrombin was as low as 0.06 pmol/L, and the detection of common interfering proteins was highly specific.

In our work, N-doped carbon dots (CDs) were synthesized by a facile hydrothermal method with fermented rice as the carbon source. The CDs show bright blue fluorescence, and the maximum emission wavelength was 380 nm with wavelength ranges from 320 to 560 nm. Interestingly, these as-prepared CDs show strong blue photoluminescent properties under the radiation with ultraviolet (UV) light. Moreover, it also exhibits good sensitive fluorescence detection for Fe³⁺ ions; the detection limit is 0.1 μM, which is significant fluorescence quenching based on CDs. Other representative metal ions were further tested to verify their selectivity, which provides a solid underpinning for the practical use in Fe³⁺ ions detection in real samples, e.g. underground water. In addition, the CDs work well as a fluorescent ink and can encrypt and store information.

An efficient method based on high-performance liquid chromatography coupled with atomic fluorescence spectrometry (HPLC-AFS) was successfully developed for the simultaneous determination of four mercury species including Hg²⁺, methylmercury (MeHg), ethylmercury (EtHg), and phenylmercury (PhHg) in water. Samples were enriched and cleaned up with a solid-phase extraction (SPE) pretreatment using a thiol cartridge. Some key parameters including the selection of a SPE cartridge, eluent type, eluent volume, and interference factors were systematically investigated. Chromatographic separation was achieved on a C₁₈ column using a mobile phase consisting of methanol and 60 mmol L⁻¹ ammonium acetate with 10 mmol L⁻¹ L-cysteine by gradient elution. Under the optimized conditions, good linearity (r ≥ 0.9991) was observed between 0.20 to 10.0 μg L⁻¹. The limits of detection were in the range of 0.001 – 0.002 μg L⁻¹. High recoveries (87.2 to 111%) and good reproducibility (1.1 – 6.5%) were obtained. Such a method is sensitive, selective and accurate, which can be applied to the quantification of mercury species in water samples.

A method for the simultaneous determination of arsenobetaine, arsenite, arsenate, dimethylarsinic acid, monomethylarsonic acid, selenite, selenate, bromate, bromide, iodate, and iodide in bottled drinking water and fruit juice samples was established by using high-performance liquid chromatography–inductively coupled plasma mass spectrometry. The separation of eleven compounds was performed on an ion exchange chromatography column (Dionex IonPac AS14) with 20 mmol L⁻¹ (NH₄)₂CO₃ (pH 10) and 50 mmol L⁻¹ (NH₄)₂CO₃ (pH 10) as a mobile phase. The limits of quantification of the method were 0.17 – 1.2 μg L⁻¹ for the test compounds in bottled drinking water and 0.34 – 2.4 μg L⁻¹ in fruit juice. The average recoveries ranged from 85.8 to 102.2%, and the relative standard deviations (RSDs) obtained in fortification recovery studies were generally <4.2% for bottled drinking water samples. The average recoveries ranged from 88.1 to 118.0% (except for iodate) for fruit juice sample.

An electrochemical sensor for phosphate detection in body fluids was developed based on the hydration transition of magnesium hydrogen phosphate (newberyite, MgHPO₄·3H₂O). The sensor was fabricated through incubation of a multi-walled carbon nanotube/Nafion (MWCNT/Nafion) modified glassy carbon electrode (GCE) in magnesium phosphate solution, where MgHPO₄·3H₂O was self-assembled on the electrode surface (denoted as MgP/MWCNT/Nafion). An electrooxidation peak at 1.0 V vs. Ag/AgCl was observed when the as-prepared electrode was subjected to a differential pulse voltammetry (DPV) scan in the presence of phosphate in acetate solution. When the DPV scan was performed in 0.4 – 1.3 V vs. Ag/AgCl, a linear relationship was observed between the peak height and the phosphate concentration in the range from 0.01 to 25 μM in the presence of 0.1 mM Mg²⁺ in the acetate solution with a limit of detection of 32 nM. And the sensor was successfully applied for phosphate detection in human urine and saliva samples with recoveries of 94.7 – 104.4 and 96 – 103.3%, respectively.

The present work studied an acclimation method for phosphorus accumulating organisms (PAOs) with a high content of acetone in culture solutions to develop microbial-based enzyme sensors for highly hydrophobic organophosphorus (OP) pesticides. Through three steps of cultivation and acclimation, only rod-shaped bacteria survived among the various PAOs. The extracellular enzymes released from the acclimated PAOs were salted out by using ammonium sulfate, then purified by a dialysis membrane and a DEAE-Sepharose FF anion exchange column. Two enzyme components were successfully separated—both of which showed hydrolase activity on disodium p-nitrophenyl phosphate (enzyme I, 1.57 μmol/(min·μg); enzyme II, 0.88 μmol/(min·μg) at 45°C). Further, SDS-PAGE gel electrophoresis results showed that the molecular weights of enzymes I and II were about 15.11 and 11.98 kDa, respectively. On this basis, the applicability of the enzyme in hydrophobic OP biosensors was demonstrated.

A binary amorphous polymer blend consisting of polyvinyl chloride (PVC) and polymethyl methacrylate (PMMA) was studied with a rheo-optical characterization technique based on the combination of a near-infrared (NIR) spectrometer and a tensile testing machine. In rheo-optical NIR spectroscopy, tensile deformations were applied to polymers to induce the displacement of molecular chains while being probed by NIR light. The application of this technique was extended to a partially miscible amorphous polymer blend consisting of PVC and PMMA to demonstrate how it can be utilized to detect subtle but important deformation behavior. A change in the NIR spectral feature revealed that the initial deformation of the blend induces the reorientation of the PVC chains. A part of the PMMA connected to the PVC was tagged during the PVC deformation. Further deformation of the sample eventually resulted in necking propagation to the surrounding area.

A novel electrochemical glassy carbon electrode modified with a multi-walled carbon nanotube, cytochrome c (Cyt c) and zinc oxide nanoparticles (ZnONPs) was fabricated to increase the sensitivity of electrode for the detection of streptomycin (STN) in certain pharmaceutical samples. Cyclic voltammetry (CV) and differential pulse voltammetry techniques were used for an electrochemical characterization of the electrode. Furthermore, the electrochemical biosensor construction phases were examined by using X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier-transform infrared spectroscopy (FTIR). Under the optimal experimental conditions, the electrode offers a high selectivity and sensitivity signaling in the co-existence method of STN with the linear concentration ranging from 0.02 to 2.2 μM. The detection limits (LOD) and limit of quantification (LOQ) were found to be 0.0028 and 0.0562 μM, respectively. The fabricated sensing electrode has good stability, reproducibility and sensitivity towards STN in the pharmaceutical samples. Preliminary determinations of binding sites within the specified grid box size, which covers both Cyt c and STN, were done by molecular docking analysis. Moreover, density functional theory (DFT) computations were performed to provide insightful information into the optimized geometry of STN.

Hydrogen peroxide (H₂O₂) is a main reactive oxygen by-product produced in the metabolism of organisms and a common biomarker of oxidative stress. Aggregation-induced emission (AIE) probes for H₂O₂ have been proposed. Such AIEgens mostly use benzeneboronic acid as a recognition group. Recently, a strategy involving enzyme-catalyzed polymerization of AIE compounds shows great potential in AIEgens design. We herein modify the AIE motif, tetraphenylethene (TPE) with o-phenylenediamine (TPE-TAF), which can be oxidated by H₂O₂ in HRP to form an intramolecular phenazine structure. Compared with a similar approach, the proposed strategy is simple and the TPE-TAF showed a sensitive “turn-on” fluorescence with H₂O₂. The detection limit (LOD) is 3.39 μM and the probe is highly specific against H₂O₂. We further verified the reaction mechanism of the enzyme-catalyzed coupling reaction. The probe is a promising candidate as a stable and safe fluorescent substrate in H₂O₂ sensing.

We developed and validated a liquid chromatography–electrospray ionization–time of flight/mass spectrometry method for the determination of abiraterone (Abi) and its metabolites (Δ⁴-Abi, 3-keto-5α-Abi, 3α-OH-5α-Abi and 3β-OH-5α-Abi) in human serum using Abi-d₄ as the internal standard. As a pretreatment procedure of serum samples, solid-phase extraction based on a silica-gel cartridge was used. The relative recovery of Abi and its metabolites was over the ranges of 84.5 – 109.2% at a concentration of 6.0 ng mL⁻¹ for Abi and 0.6 ng mL⁻¹ for its metabolites. The method was free from matrix effects. The calibration curve of Abi was linear over the range of 2.0 – 400 ng mL⁻¹ and those of its metabolites over the ranges 0.2 – 40 ng mL⁻¹. The results of the intra- and inter-day accuracy and precision data were within the FDA acceptance criteria. The optimized method was applied for the determination of Abi and its metabolites in human serum after oral administration of Abi acetate.

The formation of self-assembled monolayers (SAMs) is a normal method for the immobilization of biorecognition elements immobilized on SPR sensors. With this method, mercaptopropionic acid (MPA) with carboxylic and thiol group is the most commonly used. Dimercaptosuccinic acid (DMSA) having two carboxylics and two thiol groups is a classical antidote for heavy metal ions. In this paper, DMSA was first used to form SAMs to connect the antigen on the chip of a surface plasmon resonance (SPR) immunosensor for detection of salbutamol (SAL), and the results were compared with a traditional (MPA)-SPR sensor. Dihydrolipoic acid(DHLA)-SPR showed that the recognition efficiency of antigen and antibody of DMSA-SPR immunosensor was 170.1% at room temperature with the linear range of 5 – 150 ng/mL. The recovery rate of this sensor applied to SAL detection in pork reached 94.9 – 108.0% and the limit of quantification (LOQ) was 5 ng/mL. The results were in good correlation with the analysis results of ultra-high phase liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) analysis. This novel DMSA-SPR immunosensor provides insight into a new idea and method for improving the sensitivity of SPR immunosensors and can be widely used in the detection of other small molecules.

The use of a metal mesh device (MMD) as a precision bacterial separation filter is described. The MMD uses a structure in which identically shaped pores are arranged in a thin metal membrane. Four types of MMD with different pore sizes were used to fractionate bacteria in two types of soil. Through metagenomic analysis, the distribution of bacteria in the soil samples and in each MMD fraction was examined. In addition, eight types of previously described organic compound-degrading bacteria were used to evaluate the method, and changes in their composition following MMD fractionation were investigated. It was found that MMD fractions were enriched for all eight bacteria when compared with the initial sample. These results suggest that bacterial fractionation using MMD can enrich bacteria occurring at low frequencies in environmental samples.

An in-hospital rapid method for quantifying the serum level of favipiravir (FPV) in the pharmacological treatment of COVID-19 was developed by an appropriate combination of a solid-phase extraction treatment and a reversed-phase HPLC/UV detection system. The quantification method was well-validated and applied to measuring the serum FPV level in a clinical practice at a general hospital that accepts COVID-19 patients. Furthermore, an analysis of data from our preliminary interaction analysis revealed, for the first time, that FPV selectively forms complexes with ferric (Fe³⁺) and cupric (Cu²⁺) ions.

Silver or palladium shelled gold nanoparticles were fused into alloy nanoparticles by pulsed-laser irradiation. The alloy nanoparticles could carry antibodies on their surfaces without affecting their immune functionalities and interact selectively with antigens on a blotting membrane. Silver or palladium ions desorbed from the alloy nanoparticles as reporter ions upon the UV laser irradiation in a mass spectrometer.

An electrochemiluminescence (ECL) analytical device was developed using an electric contactless power transfer system. A mutually induced electromotive voltage was generated by wrapping an enameled wire around a commercial contactless charger. There was no electrical contact between the power supply and the electrochemical cell. For the tris(2,2′-bipyridine)ruthenium(II) (Ru(bpy)₃²⁺)/tri-n-propylamine system, a weak ECL signal was observed. When an inexpensive rectifier diode was introduced between the coil and the working electrode, the ECL intensity detection sensitivity increased by more than 100 times. The relationship between the waveform of the applied voltage and the ECL response was clarified, and the optimum conditions were determined. The intensity of the induced electromotive voltage was easily controlled by changing the number of turns in the coil. The proposed method is a safe, simple, and inexpensive technique without electrical contact.