A simple and rapid flow-injection method is reported for the determination of retinol, its derivatives and
α-tocopherol by its enhancement effect on a potassium permanganate-formaldehyde chemiluminescence system in an acidic medium. Linear calibration graphs were obtained between 5.0 × 10
-8 to 5.0 × 10
-6 mol L
-1 for all-
trans-retinol, 5.0 × 10.8 - 2.5 × 10
-6 mol L
-1 for all-
trans-retinal, 5.0 × 10
-7 - 1.0 × 10.5 mol L
-1 for retinoic acid, 1.0 × 10
-7 - 5.0 × 10
-6 mol L
-1 for retinyl acetate and retinyl palmitate and 1.0 × 10
-8 - 5.0 × 10
-6 for
α-tocopherol, with relative standard deviations (RSD;
n = 4) in the range of 0.8 - 2.8%. The limits of detection (
S/
N = 3) were in the range of 5.0 × 10
-9 - 1.0 × 10
-7 mol L
-1 with a sample throughput of 100 h
-1. The influence of common ions, excipients in pharmaceutical preparations and related organic compounds were studied on the determination of retinol and
α-tocopherol individually. The method was applied to determine retinol and
α-tocopherol in pharmaceutical preparations, and the results obtained were in reasonable agreement with the amount labelled.
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