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Article type: Cover
2000 Volume 70 Issue 2 Pages
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Published: February 01, 2000
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Article type: Appendix
2000 Volume 70 Issue 2 Pages
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Article type: Index
2000 Volume 70 Issue 2 Pages
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Article type: Index
2000 Volume 70 Issue 2 Pages
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Atsuo Katai, Takuma Furitsu, Isao Nagai
Article type: Original Contribution
2000 Volume 70 Issue 2 Pages
53-55
Published: February 01, 2000
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We used polymerase chain reaction(PCR) method to detect Verocytotoxin-producing Escherichia coli(VTEC) and evaluated this method in view points of simplicity, promptitude and economy. Commercial common primers were used. These results were evaluated as follows. 1.When 10μl samples contain over 1, 500CFU, this test method was available. 2.Cost of this test was \130 per one sample. Twenty five minute were necessary to deal with ten samples. 3.It look 3〜4 hours to judge the result of the test after the start of bacterial cultivation. 4.Because the liquid culture medium inhibits the PCR reaction, it was necessary to use sterilized water to dilute the samples. As the VTEC is getting various serotypes recently, the PCR method will become one of the good method to detect the VTEC rapidly.
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K. Nakahara
Article type: Review
2000 Volume 70 Issue 2 Pages
56-
Published: February 01, 2000
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N. Tatsumi, [in Japanese], [in Japanese]
Article type: Review
2000 Volume 70 Issue 2 Pages
57-61
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S. Kamei
Article type: Review
2000 Volume 70 Issue 2 Pages
62-67
Published: February 01, 2000
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Y. Uchiyama
Article type: Review
2000 Volume 70 Issue 2 Pages
68-73
Published: February 01, 2000
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Y. Sugioka, [in Japanese], [in Japanese], [in Japanese]
Article type: Review
2000 Volume 70 Issue 2 Pages
74-79
Published: February 01, 2000
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M. Maekawa
Article type: Review
2000 Volume 70 Issue 2 Pages
80-84
Published: February 01, 2000
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T. Fuse, [in Japanese], [in Japanese]
Article type: Review
2000 Volume 70 Issue 2 Pages
85-91
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M. Nishibori
Article type: Review
2000 Volume 70 Issue 2 Pages
92-97
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Y. Kojima, [in Japanese], [in Japanese], [in Japanese]
Article type: Experience to Use
2000 Volume 70 Issue 2 Pages
98-101
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M. Kaufman
Article type: Report
2000 Volume 70 Issue 2 Pages
102-105
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Article type: Appendix
2000 Volume 70 Issue 2 Pages
106-107
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Article type: Appendix
2000 Volume 70 Issue 2 Pages
108-
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Article type: Appendix
2000 Volume 70 Issue 2 Pages
A10-A11
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Article type: Appendix
2000 Volume 70 Issue 2 Pages
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Article type: Cover
2000 Volume 70 Issue 2 Pages
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