We evaluated the effect of rearing temperature (13 and 17°C) on the toxicity of Poly(I:C) against Japanese flounder
Paralichthys olivaceus (mean BW 8.2 g). In the fish reared at 17°C, we observed signs of toxicity such as redness or ulceration of the skin around the inoculation site or death in 41.7%, 16.7%, 16.7% and 0% of the fish injected with 800, 400, 200 and 100 μg Poly(I:C)/fish, respectively. In the fish reared at 13°C, we observed signs of toxicity in 83.3%, 75.0%, 25.0% and 33.3% of the fish that were injected with 800, 400, 200 and 100 μg Poly(I:C)/fish, respectively. Our results suggest that the toxicity of Poly(I:C) is higher in Japanese flounder reared at 13°C than in those reared at 17°C.
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