The results of previous studies on carbohydrate utilization of trout were not necessarily agreed. Also, no study on the carbohydrate utilization of marine fish has been attempted up to date. Therefore, the present paper deals with the water soluble vitamin requirement of red sea bream (Chrysophyrys major) fingerling on the feeding of high glucose and diet with the glucose utilization on the administration of the enough quantity of vitamin. The results obtained can be summarized as follows: (1) The growth of fish fed the 35% glucose diet containing the intact modified HALVER'S vitamin supplements (Table 1) was similar to the growth of fish fed the diet containing the doub lewater soluble vitamins or the fourfold (Fig. 1). Therefore, it was concluded that the intact vitamin supplements used is sufficient for the growth of red sea bream fed the high glucose diet. (2) No appreciable difference between the growth of fish fed the diets containing zero and 10% level glucose was observed. The fish fed zero and 10% level glucose had more growth and higher feed efficiency than 30% and 40% level groups (Table 2, Fig. 2 and Table 3). (3) The digestibilities of protein and glucose decreased in proportion to the increased conten tof glucose in the diet (Table 3). (4) The percentage liver weight increased as the glucose content in diets increases. The glycoge ncontent in muscle and the fat content in liver of fish fed 40% level glucose were highest (Table 4-1). (5) All the groups had similar values of hemoglobin (Fig. 3). The fish fed 40% level glucose diet had the high values of blood sugar, acetone body in blood and the low value of total cholesterol in serum as compared with the fish of other groups (Fig. 4, 5 and 6). (6) It is considered that the low rates of growth and feed efficiency by the high glucose levels are caused by the low digestibilities of protein and glucose in diets. Also, it is suggested that the cause of these low rates is the low utilizability of fish for glucose absorbed, judging from the hepat-hypertrophy, the marked accumulation of glycogen into liver and muscle, the incre asedblood sugar and acetone body in blood of fish fed high glucose diets. This utilizability may benot improved by the addition of a enough quantity of water soluble vitamins closely connected with the carbohydrate metabolism.
Outbreaks of furunculosis of salmonid fish are often observed at hatcheries in Hokkaido, Japan, when the fish were stored untill to mature. Usually, the outbreaks have been believed to be due to the infection of Aeromonas salmonicida. The author isolated an etiological agent from the infected fish. To determine the taxonomic position of the isolate, a detailed study was made on morphological and physiological properties. As the results, the isolate was found to be a non-motile Aeromonas. It was different from A. salmonicida or A. salmonicida subsp. achromogenes on the basis as shown in Table 4, to warrant its designation as a new subspecies of A. salmonicida. (In the next paper, part 2, the name of this subspecies will be proposed.)
In the previous paper, the organism was designated as a new subspecies of Aeromonas salmonicida according to the morphological and physiological properties. In this study, the serological relationship between this organism and related oranisms was investigated. As the results, the organism was clearly crossed to A. salmonicida in the heat stable antigenicity, but the both were different from each other in the heat labile and extracellular antigenicities. The present author's proposition of a new subspecies of A.salmonicida for the organism was warranted by the above results. Therefore, the author designated the organism as Aeromonas salmonicida nov. subsp. masoucida.