Changes in bilirubin contents and composition in the bile of starved and CCl
4 injected carp Cyprinus carpio were examined by diazo methods. The doses of CCl
4 injection were 10, 40, and 100μl/100g body weight.
In the bile of control carp, total, conjugated, and unconjugated bilirubin contens were 0.03-0.13, 0.02-0.08, and 0.01-0.02 mg /ml, respectively. During the starvation, total and conjugated bilirubin contents gradually increased but unconjugated bilirubin contents did not change. After 30 days, total and conjugated bilirubin contents were 22-26 times higher than those of control.
In the bile of CCl
4 injected carp, total and conjugated bilirubin contents increased rapidly. Both contents were 8-18 times higher than those of control one day after injection. Unconjugated bilirubin contents in carps injected with 40μl and 100μl CCl
4 were increased 5 days and 2 days after injection, respectively.
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