To understand the functional roles of microorganisms in aquaculture, the interaction between the microalga
Nannochloropsis oculata and the ciliate
Euplotes sp., which occurs in mass culturing of the rotifer
Brachionus plicatilis, was examined. We carried out culture experiments of
Euplotes sp. initially inoculated at densities of 1 cell/drop 0.15ml, 30 cells ml
-1, and 400 cells ml
-1.
Euplotes sp. were fed dietary bacteria together with
N. oculata (initial density; 1×10
7 cells ml
-1), the algal culture filtrate, or no algae (control experiment).
N. oculata at a density of 1×10
7 cells ml
-1 or more clearly suppressed the growth of
Euplotes sp. in all experiments. The final yields were 20∼49% of the controls. However, the culture filtrate of
N. oculata did not suppress the growth of
Euplotes sp. On the contrary, the co-existence of increased
Euplotes sp. of ca. 1000 cells ml
-1 or more distinctly caused the algae to aggregate, and inhibited the algal growth by 80% of the final densities. Consequently, antagonistic interactions, appearing to be density-dependent, were found to occur between these microbes. To our knowledge, this is the first report on the anti-ciliate activity of the algae.
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