Growth of seeds after a given period of time of germination was found to vary with the time of inoculation or sowing. Observations of growth patterns were made using seeds of two kinds of plant, one of which was a kind of barnyard grass and the other a kind of rape.
The growth rate was likewise altered depending on the day and season the observation was made.
The growth rate was influenced by the material or the substance by which the seeds were covered. Even when the material or the substance was present near them the effect could be demonstrated.
All these phenomena were previously found when Proteus vulgaris was employed in the study. The effect of time and place upon the growth was, however, never always equal between the bacteria and the seeds; that is, a condition favorable for the bacterial growth was not always beneficial to the growth of seeds and vice versa. This was also demonstrated between the two kinds of seeds.

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Data are presented to show that intratumoral injection of living hemolytic streptococci could definitely cause the destruction of solid tumor of Ehrlich carcinoma in mice.

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The histochemical follow-ups of the cellular localization of the antigens of Salmonella enteritidis in the mouse tissues after the infection of a virulent strain was studied, employing double-layer method of fluorescent antiglobulin technique (Coons). After a large inoculum injection (1mg of the organism), the localization of the bacterial antigens was principally the same as observed using killed vaccines. They were found in the Kupffer cells, in the leukocytes which infiltrated around the sinusoids in the liver, and in the large round mononuclear cells of the red pulp in the spleen. However, mice died within 24 hours and the observation could not be continued any further.
Following a small inoculum injection (10-8mg of the organism-approximately 30 cells), the mice died in 6 to 14 days. The bacterial antigens were traced up to the 9th day. They were not readily demonstrated within 48 hours in sections of the liver and the spleen. On the 3rd day, the rod-shaped bacterial antigens appeared in the liver and in the spleen. On the 7th or 8th day, when the granulomas (typhomas) were formed, the antigens, in the form of rods and particles, were concentrated in their cells as well as in the Kupffer cells and in the leukocytes which infiltrated around the sinusoids. The amount of the observable antigens decreased after 9 days. The possible explanation of this phenomenon, particularly in reference to the role of antibody, was discussed.
The amount of bacterial antigens present corresponded considerably well with the number of viable organisms obtained culturally, particularly in the early period of the infection (within 5 days).
These bacterial antigens in the form of rods were not observed in the sections stained by the conventional histological method. The staining properties of the bacilli and the difference in the size indicated that the status of the bacilli in the animal was substantially different from that observed in vitro.
As described in Discussion, the results of the present experiments support the idea that the typhomas may be formed by the antigen-antibody reaction of Salmonella antigens.
It was proven by this study that fluorescent antibody method is a useful tool of demonstrating the parasites in the host, which cannot be observed by conventional staining techniques.

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In the cultures containing charcoal, maximum yield of butanol was produced by Cl. butyricum (b) and Cl. pasteurianum (d) after 15 and 22 days of fermentation respectively. The formation of acetone was maximum in the culture of Cl. butyricum (b) after 15 days, and in the culture of Cl. acetobutylicum (f) after 22 days. In the culture containing no charcoal maximum butanol yield was obtained by Cl. acetobutylicum (f), and the yield of acetone was maximum in the culture of Cl. butyricum (a) after 15 days of fermentation. After 22 days, the analysis showed maximum butanol yield in the culture of Cl. pasteurianum (d), and the yield of acetone was maximum in the culture of Cl. butyricum (a).

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Antigenic analyses of five species of the genus Debaryomyces, including D. hansenii var. membranaefaciens and Schwanniomyces occidentalis, were carried out by the slide agglutination method, using monospecific antisera and absorbed antisera. The antigenic structures of every species examined were confirmed by the reciprocal absorption and the comparative study was made against many stock cultures and isolates.
Debaryomyces hansenii possesses the thermostable antigens 1, 2, 3, 4 and 14 and the group specific antigen 9 of C. guilliermondii, but no thermolabile antigen was found. The antigenic structures of D. kloeckeri, D. nicotianae and D. hansenii var. membranaefaciens are identical in every respect to that of D. hansenii.
Schwanniomyces occidentalis contains the same antigens present in D. hansenii in respect to thermostable antigens, but it has the thermolabile antigen j. It can be placed in Group V on the basis of having the group specific antigen 9.Debaryomyces globosus has the thermostable antigens 1, 3, 10, 24 and 29 and thermolabile antigen i. It can be placed in Group VII of S. rosei on the basis of having the group specific antigen 24, but it should be regarded as an independent species because of the distinct serologial differences from that species.
In the comparative study, the antigenic structures of many strains from each recognized species coincided with those of the standard strains when analyzed. onsequently, they will serve as the reliable basis in the classification of the species.

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An Actinomyces strain was isolated from a tooth pulp of a 36 year-old patient who was not aware of its affection. This strain was identified as A. israelii by the sugar fermentation pattern and colonial morphology, employing the nomenclature of oral Actinomycetes proposed by Onisi and Tokiwa⁽¹¹⁾.

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