1) Four strains of Pseudomonas aeruginosa, Nos. 134, 145, 117 and 58, with different pigment producing ability on glycerol-agar medium, were cultivated on glutamic acid medium to observe their mode of pigment production.
2) The glutamic acid medium developed by the authors is of a simplecomposition, and the production of pigments and fluorescent substances in this medium is so rapid and brilliant that it is valuable in the study of pigment production by the Pseudomonas.
3) Four strains of Ps. aeruginosa were grown in the glutamic acid medium, and gel filtration was carried out on their culture filtrate. The following results were obtained.
a) The fluorescent material produced by Ps. aeruginosa can be separated into at least two portions on the dextran gel column. They were designated as Fluorescence I and Fluorescence II.
b) It is not clear whether the greenish yellow part of Fluorescence I appearing in the early stage of cultivation of strains No. 134 and 145 is or not identical to Fluorescence I.
c) The red and orange pigments of strain No. 145 and the blue pigment of No. 58 are believed to be of different substances.

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Effects of pre-irradiation treatments on the ultraviolet-induced mutation frequency and survival were investigated in an avirulent strain (Jucho strain) of Mycobacterium avium.
Pre-irradiation incubation in phosphorus-deficient medium gave rise to an increase in sensitivity to ultraviolet irradation when this pre-treatment itself caused no viability damage. Post-irradiation incubation in a medium supplemented with phosphates did not recover the loss in viability caused by ultraviolet irradiation in the cells pre-incubated in phosphorus-deficient medium.The increase of ultraviolet sensitivity was compatible with the previous finding in that ultraviolet irradiation produced a significant liberation of radioactive phosphorus compounds during irradiation.
Pre-irradiation incubation of cells in phosphorus-deficient medium promoted a marked decline in ultraviolet-induced mutation frequency to streptomycin resistance, while it caused no decline in induced mutation frequency to isoniazid resistance. There was a marked discrepancy between the effects on these two mutations.
It is supposed that phenotypic expression of mutation to streptomycin resistance requires sufficient amount of some phosphorus compounds.
Pre-irradiation incubation in nitrogen-free medium also caused increase in ultraviolet sensitivty.

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Stools from 1913 apparently normal human were examined for the presence of Hafnia, and it was found that 13per cent of the individuals examined shown to be carriers of Hafnia.
The biochemical characteristics of the 327 Hafnia cultures isolated from normal human were almost uniform, and they were similar to those described by previous investigators.
From the results of serological studies, 51 O antigenic groups and 27H antigens have been demonstrated among these 327 Hafnia cultures, and according to the combinations of their O and H antigens they were classified into 144 serotypes which involved 130 hitherto undescribed new serotypes.
During the course of these serological studies, 32 new O antigenic groups and 9 new H antigens were established, and they had been assigned to as O30 to O61 and H24 to H32, respectively.
The pathogenicity of Hafnia for human and the serotypes of the Hafnia in normal human were discussed, and it was concluded that probably a majority of the Hafnia would not be pathogenic for human.

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