The physical properties of damaged hair such as break point and extention were studied in relation to Cu-uptake onto hair.
It was found that;
(1) A good relationship existed between Cu-uptake and degree of damage in a bleached or in a cold waved hair. The Cu-uptake onto hair increased with the increase of the degree of damage. It may be possible to evaluate the degree of damage in hair by Cu-uptake as a parameter.
(2) There was a good relationship between Cu-uptake and the break point or the extention of damaged hair. With the increase of Cu-uptake onto hair, the break point of damaged hair decreased, while the extention of damaged hair increased. These physical properties of damaged hair did not depend on a chemical treatment.
(3) The break point of damaged hair had the maximum point at the range of pH 4 to 6, which seems to be related to the isoelectric point of keratin fiber.

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The mutual separation of non-ionic surfactants containing polyoxyethylene (POE) chain was accomplished by high-performance liquid chromatography (HPLC) by using a UV detector after the esterification with 3, 5-dinitrobenzoyl chloride. The molar absorption coefficients (ε) at 254nm of the benzoylates of these materials having different POE chain length are confirmed to be practically identical (1.1×10⁴) by measurments of ε of the homogeneous polyethylenglycol monododecyl ethers, and following conclusion can be established that these chromatograms should give the molar distribution of POE chain length directly.
Further, the statistical treatment of the chromatograms has been studied and then concluded that this HPLC analysis is one of the most convenient method for routin analysis of nonionic surfactants containing POE chain. Two typical molar distribution of POE chain length (We-ibull Nycander and Poisson distribution) were analyzed precisely by this HPLC method.

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Chemotaxis of the slime mold Physarum polycephalum for various cosmetic ingredients was investigated in relation to their effects on the skin using double-chamber method.
The effect of these agents on the slime mold appeared in the far lower concentration range than at general safety evaluation tests for animals such as guinea pigs and rabbits.
The slime mold exhibited negative chemotaxis for all the cosmetic ingredients tested.
The relationship was recognized between the threshold concentration of cosmetic ingredients for chemotaxis of slime mold and their toxicity (LD₅₀ etc) in general test animals.

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The particle size of an emulsion plays an important role both in the long-term stability for storage and in the texture in application to skin and/or hair. It is very important to predict the particle size changes of emulsions in ageing.
This paper, at first, shortly surveys on the accelerated tests for emulsion stability, then presented the results of the particle size changes of emulsions in ageing by means of Particle Analyser. It came to conclusions that;
(1) Particle Analyser is a simple and convenient technique, which gives us much informations of emulsion stability.
(2) An empirical relationship existed between average particle size (P) and elapsed time (T). That is, LogP=kLogT+C where k is an apparent rate of coalecence and C is a constant.
(3) The degree of separation increased with the increasing of k. The apparent rate of coalescence, k, seems to be a useful index for emulsion stability.

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Alkaline phosphatase was microcapsulated to be immobilized with stylene polymer by employing a drying in liquid method. The size distribution of the microcapsules was a bimodal form. The larger microcapsules had the stronger activities and were the more dense than the one with the smaller size. The optimum temperature and pH for the reaction of the microencapsulated enzyme shifted to the higher ranges than the native enzyme. The reaction rate of the microcapsules because delayed significantly. The reaction process was analyzed by Lineweaver-Burk plots of data to test the Michael-Menten equation. It was found that the reaction rate was controlled by not only the diffusion of donor in the microcapsule but also a cappillary flow of the enzyme solution through the pin holes on the surface of the microcapsule, which were observed by a scanning electron microscope. The latter was proved by the fact that the reaction rate was affected by agitation speed and the reaction proceeded still more even if the microcasules were removed from the system.

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The quantitative determination method for arsenic (As), lead (Pb), and Cadmium (Cd), in cosmetics has been designed. In this method, the sample solutions are prepared by a wet decomposition method, with nitric acid-sulfuric acid- perchloric acid and a dry ashing method.
As, Pb and Cd are determined by the flame atomic absorption spectrophotometry (FAAS). As and Pb are also determined by the gutzeit method and the dithizone method respectively.
In this paper, recovery rate of these metals from several types of cosmetics are examined. The results indicate the FAAS is most suitable for its readiness and accuracy, as a determination method. The gutzeit method and the dithizone method are also applicable to cosmetics.
The wet decomposition method is successfully applied to almost all the types of cosmetics. In this case, the recovery rates of these metals are over 90%
But, in the case of Pb determination from the cosmetics which will include significant amounts of multi valent metal salts, the decomposition ought to be carried out without surfuric acid.

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Properties of diglycride as emulsifier were studied. We found HLB value of commercial glyceride containing some compounds (monoglyceride, diglyceride, triglyceride etc.) became lower with increasing content of monoglyceride. We realized polar monoglyceride have lower HLB value than diglyceride by PIT and EIP methods. Monoglyceride, had more surface active properties, more lowering effect of PIT and stronger tendency to favor W/O emulsification than diglyceride. We found diglyceride had usefull properties as cosmetic ingredient, because oil phase containing diglyceride could be emulsified easily with little water soluble emulsifier and viscosity of these emulsions were not change for three month.

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Phase diagrams have been determined as a function of temperature for the following binary and ternary systems containing molecularly distilled or commercial polyoxyethylene (n) oleyl ether (abbreviation; OL-n(m) and OL-n, respectively); OL-6.5(m)-water system and OL-5, OL-7, or OL-8-liquid paraffin-water system. The effect of temperature on the phase equilibria is very remarkable.
The phase diagrams of ternary systems for OL-5 at 50°C, for OL-7 at 70°C, and for OL-8 at 90°C were shown to be very much alike, suggesting that the hydrophile-lipophile balance of nonionics varies with temperature. From the studies of phase diagrams, it seems reasonable to think that the curvature of the oil-water interface changes from convex through flat to concave towards water with the rise of temperature. It was confirmed by the measurement of electrical conductance that the changes in structure of the ternary system with temperature are quite continuous. The molecularly distilled nonionics were found to exhibit the lower phase inversion temperatures and the higher cloud points compared with the commercial ones of the same average ethylene oxide chain length. Also, in the case of the system containing molecularly distilled nonionics, regìon I_O where water is solubilized in the oleophilic micelle shifted to the higher temperature with increasing surfactant concentration, while region S where the surfactant crystalline phase separates from the oil phase appeared in the lower temperature region, compared with the system containing commercial nonionics.
Thus, it is clear that the difference in distribution of ethylene oxide chain length, as well as temperature, has a remarkable effect on the phase equilibria in the ternary system.

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KI-Chamber, a new patch test unit, consists of an aluminum tray, a protecting sheet, a filter paper disc and adhesive tape. This was compared to the Al-test and Finn Chamber. The results suggest that the KI-Chamber is a useful patch test unit.

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