All issues
Volume 20 (1986)
- Issue 3 Pages 161-
- Issue 2 Pages 83-
- Issue 1 Pages 5-
Volume 20, Issue 2
Displaying 1-5 of 5 articles from this issue
- |<
- <
- 1
- >
- >|
-
Junichi Koyama, Okitsugu Nakata, Yoshihiro Morikawa1986 Volume 20 Issue 2 Pages 83-91
Published: September 20, 1986
Released on J-STAGE: August 06, 2010
JOURNAL FREE ACCESSIn order to estimate the molecular weights of proteins the analytical conditions of GPC and HPLC were investigated. Under presence of 0.2% SDS and 0.5M sodium phosphate buffer (pH 7.0), the plots of molecular weight versus retention time were linear in the range of 3000 to 150000 molecular weights. Furthermore, rough coformation of proteins were surmisable by analysis of changes in GPC chromatogram after denatured with 2-mercaptoethanol.
Retention times of small peptides on reversed-phase HPLC depend on their hydrophobisity. Molecular weights of peptides could be estimated by their retention times. This method is useful to estimate the molecular weights of small peptides and for pattern analysis of hydrolyzed peptides.
Proteins could be classified by analysis of their amino acids composition because they are characteristic for each proteins.
It is very effective for analysis of proteins in cosmetics and characterizing unknown proteins by combination of these methods.View full abstractDownload PDF (1067K) -
Junichiro Umeya, Motoji Takahashi, Hideo Komatsu, Yasuhiko Machida, Sh ...1986 Volume 20 Issue 2 Pages 98-102
Published: September 20, 1986
Released on J-STAGE: August 06, 2010
JOURNAL FREE ACCESSA technique to measure the dynamic viscoelasticity of human skin in vivo is described. Usually, the measurement of skin mechanical properties presents great difficulty due to its high noise level comparison with the strain signal from the skin under the simultaneous stress. A method is described that can measure the mechanical properties of the skin using decade filters and computer with developed noise suppressing logics. This method is sensitive enough to detect the difference in visco-elasticity between sites of the body, and the differences between before and after application of lotion.View full abstractDownload PDF (1112K) -
Akira Noda, Michihiro Yamaguchi, Yasuhiko Machida, Shoji Fukushima1986 Volume 20 Issue 2 Pages 103-110
Published: September 20, 1986
Released on J-STAGE: August 06, 2010
JOURNAL FREE ACCESSTwo types of complexes (G1, G2) were presented in a stearyltrimethyl ammonium chloride (STAC) aqueous solution to which 1-hexadecanol (C16OH) was added. In the region of the molar ratio (C16OH/STAC) below 1.5, G1 and G2 coexisted, but G2 alone appeared in that of the molar ratio above 1.5.
The structure of G1 with the lower melting point than that of G2 was hydrated-crystal of STAC, which contained a small amount of C16OH, because the addition of salt such as KCl raised the melting point.
On the other hand, the melting point of G2 rose continuously by the increase of the molar ratio until 3.0. This indicated that the formation of G2 was completed at the molar ratio 3.0, in other words, the complex consisted of one molecule of STAC and three molecules of C16OH. And in the region of G2, an interesting phenomenon appeared on the structure. The multilayered vesicles were obtained by the preparation at a temperature above the melting point of G2, and the multilayered sheets at a temperature below the melting point. That is, the differences of the preparation temperature altered the configration of the G2 to the multilayered vesicles or sheets without the change of the binding ratio of C16OH and STAG.
The formation of such vesicles suggsted that the complex composed of STAC of one molecule and C16OH under three molecules having monoalkyl chain behaved like a dialkyl ammonium cation at a temperature above the melting point.View full abstractDownload PDF (5063K) -
Yasuhisa Nakayama, Izumi Horii, Kiyoshi Kawasaki, Junichi Koyama, Taka ...1986 Volume 20 Issue 2 Pages 111-120
Published: September 20, 1986
Released on J-STAGE: August 06, 2010
JOURNAL FREE ACCESSDownload PDF (1411K) -
Chikako Yomota, Keizo Nakamura, Yoshio Ito1986 Volume 20 Issue 2 Pages 121-126
Published: September 20, 1986
Released on J-STAGE: August 06, 2010
JOURNAL FREE ACCESSInteraction between cationic surfactants and natural gums were investigated with attention to conductivity, sodium ion activity, alkyltrimethyl ammonium ion activity and precipitation reaction in gum-surfactant mixed solutions. Among many kinds of gums, chondroitin sulfate (CHs) as a linear-polysaccharide with high charge density and gum arabic (Ar) as a branchedpolysaccharide with low charge density were mainly used in the present work.
Binding of CHs with surfactant occurred to be completed in a narrow range of markedly lower concentration than the CMC of the surfactants used and the binding curve was extremely steep. On the other hand, binding of Ar with surfactant occurred in a wide concentration range and the binding curve was rather gentle. With respect to the concentration at which surfactants will start to bind with gums, the concentration of decyltrimethyl ammonium bromide was ten times as large as that of dodecyltrimethyl ammonium bromide.
These results indicate that there will be some strong interactions between gums and cationic surfactants and that the degree of the interaction will depends on both the charge density of gums and the alkyl chain length of surfactants. This strong interaction between gums and cationic surfactants followed by the precipitation, is thought to be very useful for the discriminative tests of various kinds of gums.View full abstractDownload PDF (700K)
- |<
- <
- 1
- >
- >|