In the foregoing report1), it was disclosed that the electroconductivity of bacterial cell suspension killed by heat decreases while the aging and discussed that such electrical change might mainly respond to the decrease of K and Na salt content in the cell, but calcium salt might exist in a insoluble form, eventhough Curran, Brunstetter and Myers2) found that the Ca content increased in the spore cells of several aerobic bacilli examined by them. According to Guillemin and Laτson3) , and Green and Larson4) , however, the conductivity of cell suspension is to be ascribed only to the free salt which is exosmosed by heating. Thus the investigation should be proceeded by the chemical analysis of the bacterial free and fixed salts to be obtainable from younger and aged cells.
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