THE BULLETIN OF TOKYO MEDICAL AND DENTAL UNIVERSITY
Online ISSN : 2435-0761
Print ISSN : 0040-8921
Volume 37 , Issue 4
Showing 1-1 articles out of 1 articles from the selected issue
  • HIGH-PRESSURE LIQUID CHROMATOGRAPHIC ANALYSIS
    Hiroe IGARASHI, Kazuaki KAMISAKA, Hidenori MAEZAWA, Takashi UESUGI
    1990 Volume 37 Issue 4 Pages 59-69
    Published: 1990
    Released: October 02, 2019
    JOURNAL OPEN ACCESS
    Degradation of indocyanine green solution by exposure to light was studied by high pressure liquid chromatography. Indocyanine green in an aqueous medium exposed to light changed rapidly into an unknown product. The plasma clearance rate and the biliary excretion rate of the unknown product were much slower than those of indocyanine green. Spectrophotometric scan revealed that the unknown product had almost the same absorption spectrum as indocyanine green. Therefore, if degraded indocyanine green solution were to be used in a liver function test, the clearance of indocyanine green assayed by spectrophotometry would apparently be much lower than that of undegraded indocyanine green. According to fast atom bombardment mass spectrometry, the molecular weight of the unknown product was 723, whereas that of indocyanine green was 775. The analysis of rat bile after injection of indocyanine green by high-pressure liquid chromatog1aphy revealed that about 1% of the administered indocyanine green was metabolized in the rat liver.
    Download PDF (1729K)
feedback
Top