The Journal of Protozoology Research
Online ISSN : 2434-7027
Print ISSN : 0917-4427
Volume 16, Issue 1-2
The Journal of Protozoology Research
Displaying 1-4 of 4 articles from this issue
  • Trina Mercedes Perrone Carmona, Juan Garrizzo, Antonio Roschman-Gon ...
    2006 Volume 16 Issue 1-2 Pages 1-8
    Published: 2006
    Released on J-STAGE: November 28, 2019
    JOURNAL OPEN ACCESS
    The susceptibility of BALB/c, C57Bl/6, DBA/2, NMRI, NIH and CD1 mouse strains to experimental infection with a Venezuelan equine isolate of Trypanosoma evansi was examined through hemoglobin concentration, packed red cell volume, number of erythrocytes per blood µl, parasitemia, body weight, and mice survival time. The said variables seem to be suitable markers to contrast the mouse susceptibility to T. evansi experimental infections. Among the evaluated mouse strains, NMRI was the more susceptible and DBA/2 the less vulnerable to the local T. evansi isolate used in the study. The resistant mouse strains could help in the identification of factors conferring natural resistance against T. evansi, while the susceptible could be used to investigate the heterogeneity of virulence observed in this parasite.
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  • Hiroshi Bannai, Yoshifumi Nishikawa, Jin-yong Seo, Chinatsu Nakamu ...
    2006 Volume 16 Issue 1-2 Pages 9-15
    Published: 2006
    Released on J-STAGE: November 28, 2019
    JOURNAL OPEN ACCESS
    An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for the detection of antibodies against C. parvum in cattle. The sensitivity and specificity of the ELISA were evaluated with the standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) were identified as positive by the IFAT. The same samples were tested with the ELISA. The optical densities at 415 nm were compared to the IFAT results and statistically analyzed to designate a provisional cut-off point. As a result, the cut-off point was concluded to be 0.08, which was considered to be the best condition in the light of its sensitivity (80%) and specificity (73.7%). Thus, it was demonstrated that the ELISA could be a useful tool for the diagnosis of C. parvum infection in cattle under field conditions to estimate the magnitude of asymptomatic infection in a cattle population and subsequent risk to humans.
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  • Michael F. Cohen, Mark Mazzola
    2006 Volume 16 Issue 1-2 Pages 16-25
    Published: 2006
    Released on J-STAGE: November 28, 2019
    JOURNAL OPEN ACCESS
    Bacterial and protozoan populations were monitored in orchard soil amended with Brassica napus seed meal. Numbers of fluorescent pseudomonads peaked one week following incorporation of the amendment and declined thereafter, whereas populations of Streptomyces spp. increased to stable levels nearly 20-fold higher than in non-treated soil. The amoeba-flagellate Naegleria americana, isolated from the amended soil, showed a marked feeding preference in culture for fluorescent pseudomonads relative to Streptomyces spp. Amended soils showed rapid declines in RSM-derived cis-oleic acid that paralleled increases in the numbers of bacteria and proportion of bacterial fatty acid markers in the soil. Arachidonic acid, which reached peak levels one week after seed meal incorporation, was found in Pythium spp. and Mortierella spp. but not in the two most abundant protozoa isolated from soil. These data indicate that arachidonic acid is not a suitable marker for protozoan quantification.
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  • Viera Revajová, Adriana Loószová, Maria Goldová, Martin Zibrín, R ...
    2006 Volume 16 Issue 1-2 Pages 26-32
    Published: 2006
    Released on J-STAGE: November 28, 2019
    JOURNAL OPEN ACCESS
    In order to morphological study of non-specific host immune response, cross-species experimental infection of Leghorn chicks with partridge specific coccidium, Eimeria procera (106 oocyst/per birds) was done.Schizonts of E. procera were histologically observed in all the parts of small intestine and caeca of chicks.Heterophilic and lymphocytic infiltration in lamina propria, and damage of villi intestine were observed. Schizonts were studied by electron microscope after 12, 30, and 60 hours post-infection (h pi). Results demonstrated immature and structurally damaged schizonts at 12 h pi, delayed maturation of first generation schizonts at 30 h pi, and phagocytosis of parasites at 60 h pi by intraepithelial lymphocytes.
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