Japanese Journal of Water Treatment Biology

ORIGINALS

Isolation of Melamine-Degrading Bacteria from Automotive Paint Waste Water and Fundamental Research on Melamine Degradation Method

HIDEHIKO SUGIYAMA, SHUJI KAJIYA, MASAO TSUJI, TAKAYUKI KAWANO, HISAO H ...

Article type: ORIGINALS
2018 Volume 54 Issue 2 Pages 47-54
Published: 2018
Released on J-STAGE: June 15, 2018

DOIhttps://doi.org/10.2521/jswtb.54.47

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Melamine derivatives in automotive paint waste water cannot be degraded with activated sludge treatment. For the purpose of degrading residual melamine derivatives, two strains of melamine-degrading bacteria were isolated from automotive paint waste water. One strain isolated at neutral condition was identified as Microbacterium sp., the other strain isolated at acidic condition was identified as Rhodococcus sp. They could degrade melamine but couldn’t degrade melamine derivatives. HPLC analysis indicated many kinds of melamine derivatives after activated sludge treatment of automotive paint waste water. But all of them were converted into melamine with acid treatment. So acidic pre-treatment of melamine derivatives made them bio-degradable. The acid-tolerant melamine-degrading bacterium isolated at acidic condition could degrade melamine at pH5.0, so acid treatment step and melamine degradation step could be carry out simultaneously. Therefore one step treatment of melamine derivatives degradation became possible.

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NOTES

Effect of Low Temperature Treatment and Incubation Temperature on the Recruitment of Cyanobacteria Cells from the Bottom Sediments Collected at Lake Hachiro, Akita

MIHO ARAKI, KUNIHIRO OKANO, MEGUMU FUJIBAYASHI, NAOYUKI MIYATA

Article type: NOTES
2018 Volume 54 Issue 2 Pages 55-63
Published: 2018
Released on J-STAGE: June 15, 2018

DOIhttps://doi.org/10.2521/jswtb.54.55

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In Lake Hachiro, Akita, algal blooms occur every summer. Cyanobacteria such as Microcystis sp. and Anabaena sp. precipitate on the bottom sediments in autumn and overwinter. In this study, we carried out the recruitment experiments at 10℃, 15℃, and 20℃ using the bottom sediments collected in November 2016 in order to evaluate whether the precipitated cyanobacteria recruit from the sediments. While the amounts of recruited Microcystis cells tended to increase during the incubation of 10 days as the temperature increased from 10℃ to 20℃, Anabaena cells did not recruit at 20℃. In the sediment samples treated at 4℃ for 20 weeks, the amounts of recruited Microcystis and Anabaena cells at 20℃ were significantly larger than at 10℃. The low temperature treatment of sediment samples obviously promoted the recruitment of Anabaena cells, suggesting the importance of low water temperature in winter for germination and recruitment of Anabaena dormant cells. This study showed that the low temperature treatment of the sediments as well as setting of temperature condition was an important item for evaluating the recruitment of cyanobacteria from lake sediment samples.

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Comprehensive Detection of Pathogenic Bacteria in Jar Water, Community Well Groundwater, and Environmental Water in the Kathmandu Valley, Nepal

DAISUKE INOUE＊, HAYATO YOSHINAGA, BIKASH MALLA, RAJANI GHAJU SHRESTHA, ...

Article type: NOTES
2018 Volume 54 Issue 2 Pages 65-72
Published: 2018
Released on J-STAGE: June 15, 2018

DOIhttps://doi.org/10.2521/jswtb.54.65

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Microbial contamination of water sources remains a serious problem in the Kathmandu Valley, Nepal. This study was conducted to comprehensively investigate the occurrence of pathogenic bacteria in water samples collected from the valley, most of which were used for drinking and other domestic purposes. The water samples consisted of 10 samples of commercial jar water, 10 of groundwater from shallow dug wells, 3 of groundwater from deep tube wells, 1 of stone spout water, 2 of spring water, and 2 of river water. DNA microarray analysis targeting 941 different pathogenic bacterial species/groups showed the prevalence of Acinetobacter, Arthrobacter, Brevibacterium, Pseudomonas, and Legionella and the presence of biosafety level 3 pathogens in some samples, which were partially confirmed by genus-specific PCR quantification for Legionella and Brucella. Additionally, the occurrence of most pathogens detected via DNA microarray analysis was not correlated with that of fecal indicator bacteria (total coliforms and Escherichia coli). These results will aid in understanding the overall picture of pathogenic bacterial contamination and in screening important pathogens that should be investigated in detail.

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