The flora and ecology of bryophytes and the changes over two decades was studied in Chiba City and Yotsukaidou City, Chiba Prefecture. The study area consisted of seven sites representative of various urban and natural environments; nature park, agricultural area, urban park, street residential district, housing complex district, commercial district and industrial district. The total number of bryophytes species in the study area in 1975 identified 83 bryophytes species, in 1995, 78 species was recorded. The total number of bryophytes species decreased during the two decades, but the number of species at each individual site increased for most sites. Agricultural district was the only site in which the number of species decreased. Species of erect forms increased at all sites, while prostrate forms decreased sharply at the nature park and agricultural district sites. Thalloid form increased greatly at the nature park site. Erect forms remained the only type found in the industrial district site. The most commonly encounted substrata for both years was soil, followed by tree trunk in 1975 and by concrete in 1995. Four species groups were recognized. Group 1 occurred at all sites from rural to heavily urbanized area. Group 2 found from rural to moderately urbanized areas. Group 3 occurred mainly in rural areas. Group 4 restricted to natural and rural areas. A positive correlation was observed between woody vegetation cover area and number of bryophytes species. The results suggest that bryophytes are useful as bioindicators to help understand environmental changes due to urbanization.
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