In the present study we investigates the influence of the local irradiation of a portion of the maxilla on the numbers of lymphocyte subsets in peripheral blood and spleen, specifically minor population lymphocytes (γ δT cells and NKT cells). Male C57BL/6 mice at 15 weeks of age were used for the experiments. In the irradiation group, a portion of the maxilla was exposed to X-ray (2.0Gy/min, 10Gy) and we analyzed lymphocytes using flow cytometry (anti-CD3, CD4, CD8, TCR αβ, TCR γ δ and NK1.1 monoclonal antibodies), and compared the outcome to that obtained from the non-irradiation groups. The following results were obtained: 1) In peripheral blood, CD4
+SP T cells, CD8
+SP T cells, α βT cells, γ δT cells and NK cells decreased significantly on the first day and third day after irradiation. NKT cells decreased significantly on the third day after irradiation. 2) In spleen, CD4
+SP T cells, CD8
+SP T cells, α βT cells and γ δT cells decreased significantly on the first day after irradiation. NK cells and NKT cells did not change significantly after irradiation. The above results indicate that the changes in lymphocytes have a direct relationship to radiosensitivity, and the origin and distribution in lymphocyte subsets.
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