Objective : To investigate the micro structural and histomorphometrical changes in mandibular condyles in ovariectomized cynomolgus monkeys.
Material and Methods : The experimental animals were female cynomolgus monkeys (n = 24) with a mean age of 10 years, range 9-15 years. The animals were divided into 2 groups : an experimental group to be ovariectomized (OVX group) and a control sham operated group (Sham group). The animals in each group were housed under the environmental condition outlined in the appropriate guidelines from the National Institutes of Health U.S.A.
Two years post-operatively, the animals were killed and the maxillo-facial bones were dissected and fixed with 10% buffered formalin. The bone mineral density (BMD) of the mandibular condyle was determined by densitometric analysis of the lateral oblique transcranial projection radiograph. Bone X-ray microanalysis on the mandibular condyle was performed using micro computed tomography (μCT). The following parameters were examined in cancellous bone : bone volume (BV/TV), trabecular thickness and number, trabecular separation, structure model index (SMI), number of node struts, and total strut length of tissue volume. Further, cortical bone volume was additionally examined.
The condyles were immersed in Plank-Rychlo's solution for demineralization, embedded in paraffin, and used for standard light microscopic observation. Thickness of the cartilage was measured and the unit number of hypertrophic cartilage cells was counted on each coronal section.
Results : Densitometric analysis of lateral oblique transcranial projection radiograph showed that BMD of the mandibular condyle did not significantly decrease in the OVX group in comparison with the Sham group. However, CT analysis did show that BV/TV of the cancellous bone, trabecular thickness and number, SMI, number of node struts, and total strut length of tissue volume in the OVX group were significantly lower than those found in the Sham group. Further, trabecular separation in the OVX group was significantly higher than that in the Sham group. However, cortical bone volumes were similar in those two groups.
Histomorphometrical analysis on condylar cartilage showed that both thickness of the hypertrophy stratum and unit number of hypertrophic cartilage cells were lower in the OVX group than in the Sham group.
Conclusion : Bone loss and trabecular fragmentation in the monkey condyle could be induced with ovariectomy. The changes occurred only in cancellous bone, but not in cortical bone.
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