Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
ISSN-L : 0007-5124
45 巻 , 1 号
選択された号の論文の15件中1~15を表示しています
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  • Yasumasa KUBOYAMA, Kaoru MORI, Kazuo SUZUKI, Akio ISHII, Katsuichi SHU ...
    原稿種別: Original
    1996 年 45 巻 1 号 p. 15-22
    発行日: 1996年
    公開日: 2003/12/23
    ジャーナル フリー
    The changes in the nasal cavity volume of rats with age and the area exposed to nasal drops administered into the nasal cavity were investigated. Results indicated that the nasal cavity volume lineally increased as rats grew older. In 7-week-old rats, the exposed area in the case of an administration volume of 25 μl, based on practice, was naso-, maxillo-, and ethmoid turbinate and this volume was enough to expose the whole area of the nasal cavity including the ethmoid turbinate. On the other hand, in 27-week-old rats, administration volumes of 10 and 25 μl were not enough to expose the ethmoid turbinate. This indicated that the exposed area tended to become narrower in 27-week-old rats than in 7-week-old rats, but the exposed area in the case of an administration volume of 50 μl was naso-, maxillo-, and ethmoid turbinate in 27-week-old rats. In this case, the volume was enough to spread to the ethmoid turbinate. Differences in the exposed area might be caused by differences in the volume of the nasal cavity. It was also indicated that the main exposed area was the inferior meatus in the 30 min immediately after administration. At all administration volumes, however, notice should be taken of the outflow of nasal drops into the oral cavity through the nasopalatine.
  • Yuzuru KATO, Mitsuo YAMAMOTO, Jiro IKEGAMI, Shuzo OKUMURA, Takuji HARA ...
    原稿種別: Original
    1996 年 45 巻 1 号 p. 23-32
    発行日: 1996年
    公開日: 2003/12/23
    ジャーナル フリー
    Recombinant human granulocyte colony-stimulating factor (G-CSF) at a dose of 1 to 300 μg/kg/day was administered intravenously to rats daily for 13 weeks. Serum alkaline phosphatase (ALP) activity increased dose-dependently with leukocytosis. Most of the increased leukocytes were segmented neutrophils, and neutrophil alkaline phosphatase (NAP) scores were elevated markedly. Serum ALP activity correlated very well with the segmented neutrophil counts, and the coefficient of correlation was more than 0.97 in both sexes. Pathological examinations revealed splenomegaly and a marked increase in neutrophils in the red pulp of the spleen. In the spleen, phagocytosis of neutrophils by macrophages was observed. These data indicate that the increased ALP was of neutrophil origin. Serum ALP activity may be increased by the direct release of ALP from the high number of neutrophils into the blood, or by the leakage of ALP into the blood mainly from the spleen where many neutrophils are pooled and destroyed by the macrophage system.
  • Hiroshi SUZUKI, Keigo YOROZU, Toshihiko WATANABE, Masao NAKURA, Jiro A ...
    原稿種別: Original
    1996 年 45 巻 1 号 p. 33-38
    発行日: 1996年
    公開日: 2003/12/23
    ジャーナル フリー
    In vitro fertilization and embryo transfer were performed for rederivation of four strains of mice harbouring mouse hepatitis virus (MHV) and/or Pasteurella pneumotropica (P. pneumotropica). Superovulated oocytes were fertilized by preincubated cauda epididymis sperm in vitro. Fertilized eggs at 2-cell stage were transferred into the oviducts of specific pathogen free (SPF) recipients. Microbial examination of sperm and/or oocyte donors verified the presence of P. pneumotropica and/or of antibodies to MHV in all strains, but neither in the recipients nor in the offspring antibodies to MHV could they be detected. The results indicate that an in vitro fertilization-embryo transfer (IVF-ET) system is an effective and simple alternative to cesarean operation in infected mice.
  • Teruo SUZUKI, Nobuo SUZUKI, Kouji SHIMODA, Hiroshi NAGASAWA
    原稿種別: Original
    1996 年 45 巻 1 号 p. 39-43
    発行日: 1996年
    公開日: 2003/12/23
    ジャーナル フリー
    The hematological and serum biochemical values of a total of 18 pregnant female squirrel monkeys were determined during the pre-and postpartum period. Pregnancy was determined by abdominal palpation in adult females cohabiting with robust males. The mean body weight of the pregnant females gradually increased toward parturition and dramatically decreased at parturition due to delivery of the infant monkey. The red blood cell count, hematocrit and hemoglobin levels diminished toward parturition and then increased to their normal levels by week 6 after delivery. The Wintrobe constant, MCHC, did not fluctuate, but MCH and MCV values increased in late pregnancy and the early nursing periods. The mean white blood cell count varied between 65.8 and 87.3 (× 102/mm 3) during the experimental periods. The mean serum total cholesterol concentration and glutamic-oxaloacetic transaminase activity were lower in the pregnant group during the mid- and late gestation stage than in nonpregnant and nursing females. The mean serum total protein and albumin values were lower in pregnant females than in the controls. Alkaline phosphatase activity increased in late pregnancy and the nursing periods. Since the pregnant females examined gave birth to healthy newborn monkeys and nursed them normally, the hematological and serum biochemical measurements should represent the physiological values for squirrel monkeys during pregnancy and the postpartum period.
  • Hiroaki YAMAMOTO, Ichiro YAMAMOTO
    原稿種別: Original
    1996 年 45 巻 1 号 p. 45-54
    発行日: 1996年
    公開日: 2003/12/23
    ジャーナル フリー
    A tumor developed spontaneously in the subcutaneous tissue of the hind leg of a 7-month-old female ddY mouse. Light and electron microscopical examinations revealed that the original tumor was composed of an admixture of fibroblast-like and histiocyte-like cells arranged predominantly in a storiform or cartwheel pattern. The tumor cells gave positive reactions for acid phosphatase, N-acetyl-β-glucosaminidase, non-specific esterase, β-glucuronidase, alpha-1 antitrypsin and fibronectin. The original tumor was diagnosed as a malignant fibrous histiocytoma (MFH). The tumor was serially transplanted into syngeneic mice up to the 92nd generation. The tumor was also consistently transplanted into allogeneic mice of several inbred strains. The allogeneic mice used in the present study were strains having different H-2 haplotypes. During succeeding passages, transplanted tumors showed aberrant growth properties. The tumor transplanted into mice of inbred strains took well to back transplantation for mice of original strain and allotransplantation for other inbred strains. The pathological features of these transplantable tumors were basically similar to those of the original tumor. As mentioned above, a MFH developed spontaneously in the ddY mouse was consistently transplantable into both syngeneic and allogeneic mice.
  • Al Muktafi SADI, Takayoshi TODA, Hirosuke OKU, Seitetsu HOKAMA
    原稿種別: Original
    1996 年 45 巻 1 号 p. 55-62
    発行日: 1996年
    公開日: 2003/12/23
    ジャーナル フリー
    To compare the atherogenecity of different fats and oils, a total of forty, 40-day-old male Japanese quails were fed one of the following diets for three months: basal diet (control), a diet-containing 15% corn oil (CO) and 2% cholesterol (CH), a diet-containing 15% oleic acid (OL) and 2% CH, a diet-containing 15% perilla oil (PE) and 2% CH, a diet-containing 15% primrose oil (PR) and 2% CH. A higher plasma cholesterol concentration was found in the birds in the CO and OL groups, whereas the PE and PR groups showed a much lower level of plasma cholesterol than the CO and OL groups. In proportion to the increased plasma cholesterol, both CO and OL groups showed narrowing of the lumen of the ascending aorta and its large branches due to marked lipid-rich intimal thickening. Ultrastructural changes in the ascending aorta and its large branches were correlated with the degree of intimal thickening. The major foam cell types were macrophages and fibroblastic cells. The PE and PR groups showed the fewest lipid-rich intimal thickening lesions in their ascending aorta and its large branches. These findings suggest that the alpha-linolenic acid contained in perilla oil is less atherogenic than oleic and linoleic acid, and gamma-linolenic acid contained in primrose oil has a tendency to decrease the plasma lipid level.
  • Masashi HASHIMOTO, Masayuki FUNABA, Matanobu ABE, Seinosuke OHSHIMA
    原稿種別: Original
    1996 年 45 巻 1 号 p. 63-70
    発行日: 1996年
    公開日: 2003/12/23
    ジャーナル フリー
    The effects of high protein feeding on food and water intake, and the retention and urinary excretion of macrominerals (magnesium (Mg), phosphorus (P), and calcium (Ca)) were examined in growing cats. Seven female cats aged 4 months were fed diets containing 55% crude protein (n=4) or 29% crude protein (n=3) for 12 months on an ad libitum basis. Mineral balances were determined at 0.5, 2, 6, 10, and 12 months of feeding. The higher protein intake stimulated daily water intake and urine excretion throughout the study, although daily food intake was not affected by dietary protein levels. The urinary Mg concentration was decreased by the high protein intake, resulting from both increased urine volume and reduced excretion of urinary Mg. In contrast, the concentration and daily excretion of urinary P were increased by the high protein intake. The protein-induced increase in urinary P would not necessarily imply the increased excretion of PO43-, the anion responsible for struvite crystallization, because the dissociation of phosphate depends on urinary pH. Urinary Ca excretion was not affected by the dietary protein levels, but the high protein intake caused less retention of P and Ca as a result of enhanced urinary P excretion and lowered Ca absorption. The possibility of high protein feeding for the prevention of struvite crystallization in growing cats is discussed.
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