Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
ISSN-L : 0007-5124
52 巻 , 5 号
選択された号の論文の15件中1~15を表示しています
Original
  • Yun-Lyul LEE, Heui-Jin KIM, Mi-Sook LEE, Ji-Min KIM, Jin-Soo HAN, Euk- ...
    原稿種別: Original
    2003 年 52 巻 5 号 p. 371-375
    発行日: 2003年
    公開日: 2003/10/22
    ジャーナル フリー
    Agaricus blazei (H1 strain) was tested for its anticancer activity using a sarcoma 180 (S180) inoculation model and the changing patterns of splenocyte subsets were examined. Its hot-water extract was administered orally to ICR and KSN nude mice that were inoculated with S180. The growth of S180 was significantly inhibited in A.blazei treated groups. Pan T cells significantly increased in all treated groups compared to controls, even in KSN nude mice. Splenocyte subset changes were slightly different between ICR and KSN nude mice. This S180 inoculation model proved to be effective in screening the antitumor effect of basidiomycetes and allowed comparisons of immunological cellular changes between the mouse strains.
  • Takashi KURIBAYASHI, Makoto SHIMIZU, Tokihiro SHIMADA, Toshio HONJYO, ...
    原稿種別: Original
    2003 年 52 巻 5 号 p. 377-381
    発行日: 2003年
    公開日: 2003/10/22
    ジャーナル フリー
    Serum alpha 1-acid glycoprotein (AAG) levels were measured in healthy beagles of various ages (66 male and 74 female) by turbidimetric immunoassay (TIA), and then separately - in pregnant beagles - by single radial immunodiffusion (SRID). The first experiment revealed that serum AAG levels ranged from 40 to 960 μg/ml (mean of 322 ± 202 μg/ml) in male dogs, and from 47 to 833 μg/ml - in female dogs (mean of 316 ± 199 μg/ml), without any significant sex- or age-related variation. The second experiment, however, revealed that serum AAG levels increased in all pregnant beagles and peaked in the middle of gestation at 250-1,000 μg/ml (mean of 634 ± 246 μg/ml). In 7 of 8 dogs the AAG levels peaked about 45 days after ovulation. Despite a high value of 1,210-1,360 μg/ml being observed for serum AAG levels in 3 pregnant beagles inoculated with Staphylococcus aureus, its levels in umbilical cord blood were below the detection limit of SRID (40 μg/ml).
  • Seiichi TANAKA, Kazuaki MANNEN
    原稿種別: Original
    2003 年 52 巻 5 号 p. 383-386
    発行日: 2003年
    公開日: 2003/10/22
    ジャーナル フリー
    Stress is one of the important factors that induces reactivation of pseudorabies virus (PrV) in latently infected pigs. We established a murine model of latent PrV infection and examined the effects of mild stress treatment in order to demonstrate that this model simulates natural infection in the pig. Latently infected mice excreted PrV from the nasal cavity under stress treatments consisting of restraint, exposure to cold or transport. Similar reactions have been observed upon treatment with acetylcholine and dexamethasone. The present findings demonstrate that these kinds of mild stress reactivate the virus in murine latent infection models in a manner similar to the induction of latent infection in pigs in the field.
  • Takashi KURIBAYASHI, Tokihiro SHIMADA, Mariko MATSUMOTO, Katsuhito KAW ...
    原稿種別: Original
    2003 年 52 巻 5 号 p. 387-390
    発行日: 2003年
    公開日: 2003/10/22
    ジャーナル フリー
    Serum C-reactive protein (CRP) concentrations in healthy beagle dogs of various ages and in pregnant beagles were measured by enzyme-linked immunosorbent assay (ELISA). Serum CRP concentrations were 1.5-16.0 μg/ml (mean 7.9 ± 3.4 μg/ml) in male, and 1.8-18.9 μg/ml (mean 8.3 ± 4.0 μg/ml) in female dogs. No significant sex-related differences were observed in the values. Further, there were no significant age-related differences either. Serum CRP concentrations increased during pregnancy. The concentration of serum CRP in pregnant dogs peaked at 70.2-90.4 μg/ml (mean 77.5 ± 7.1 μg/ml) 30 or 45 days after ovulation, demonstrating two characteristic features of CRP concentration change in pregnant dogs.
  • Kanako YOSHIZAWA, Kyoko INABA, Hideyuki MANNEN, Tateki KIKUCHI, Makoto ...
    原稿種別: Original
    2003 年 52 巻 5 号 p. 391-396
    発行日: 2003年
    公開日: 2003/10/22
    ジャーナル フリー
    Despite intensive studies of muscular dystrophy of chicken, the responsible gene has not yet been identified. Our recent studies mapped the genetic locus for abnormal muscle (AM) of chicken with muscular dystrophy to chromosome 2q using the Kobe University (KU) resource family, and revealed the chromosome region where the AM gene is located has conserved synteny to human chromosome 8q11-24.3, where the beta-1 syntrophin (SNTB1), syndecan 2 (SDC2) and Gem GTPase (GEM) genes are located. It is reasonable to assume those genes might be candidates for the AM gene. In this study, we cloned and sequenced the chicken SNTB1, SDC2 and GEM genes, and identified sequence polymorphisms between parents of the resource family. The polymorphisms were genotyped to place these genes on the chicken linkage map. The AM gene of chromosome 2q was mapped 130 cM from the distal end, and closely linked to calbindin 1 (CALB1). SNTB1 and SDC2 genes were mapped 88.5 cM distal and 27.6 cM distal from the AM gene, while the GEM gene was mapped 18.5 cM distal from the AM gene and 9.1 cM proximal from SDC2. Orthologues of SNTB1, SDC2 and GEM were syntenic to human chromosome 8q. SNTB1, SDC2 and GEM did not correspond to the AM gene locus, suggesting it is unlikely they are related to chicken muscular dystrophy. However, this result also suggests that the genes located in the proximal region of the CALB1 gene on human chromosome 8q are possible candidates for this disease.
  • Hideki KATOH, Koji ODA, Kyoji HIOKI, Kaori MUGURUMA
    原稿種別: Original
    2003 年 52 巻 5 号 p. 397-400
    発行日: 2003年
    公開日: 2003/10/22
    ジャーナル フリー
    We have established a genetic quality testing system for early stage embryos of the mouse. A method of preparation of template DNA for PCR was established using the lysis buffer (1 × PCR reaction buffer supplemented with proteinase K at a concentration of 40 μg/ml) developed by the authors. We demonstrated that two 8-cell embryos of an inbred strain provide sufficient volumes of template DNA for PCR to identify the strain of embryos using four microsatellite markers (D3Mit54, D5Mit18, D6Mit15 and D8Mit50) differentiating 13 inbred strains of mice. This system will be useful in embryo banks that have recently been established worldwide for demonstrating the genetic accuracy of a given strain prior to recovery of live animals.
  • Fanglin ZHANG, Chuanzhong YE, Guo LI, Wei DING, Wenzhong ZHOU, Hongda ...
    原稿種別: Original
    2003 年 52 巻 5 号 p. 401-407
    発行日: 2003年
    公開日: 2003/10/22
    ジャーナル フリー
    To develop a rat model of type 2 diabetic mellitus that simulated the common manifestation of the metabolic abnormalities and resembled the natural history of a certain type 2 diabetes in human population, male Sprague-Dawley rats (4 months old) were injected with low-dose (15 mg/kg) STZ after high fat diet (30% of calories as fat) for two months (L-STZ/2HF). The functional and histochemical changes in the pancreatic islets were examined. Insulin-glucose tolerance test, islet immunohistochemistry and other corresponding tests were performed and the data in L-STZ/2HF group were compared with that of other groups, such as the model of type 1 diabetes (given 50 mg/kg STZ) and the model of obesity (high fat diet). The body weight of rats in the group of rats given 15 mg/kg STZ after high fat diet for two months increased significantly more than that of rats in the group of rats given 50 mg/kg STZ (the model of type 1 diabetes) (595 ± 33 g vs. 352 ± 32 g, p<0.05). Fast blood glucose levels for L-STZ/2HF group were 16.92 ± 1.68 mmol/l, versus 5.17 ± 0.55 mmol/l in normal control and 5.59 ± 0.61 mmol/l in rats given high fat diet only. Corresponding values for fast serum insulin were 0.66 ± 0.15 ng/ml, 0.52 ± 0.13 ng/ml, 0.29 ± 0.11 ng/ml, respectively. Rats of type 2 diabetes (L-STZ/2HF) had elevated levels of triglyceride (TG, 3.82 ± 0.88 mmol/l), and cholesterol(Ch, 2.38 ± 0.55 mmol/l) compared with control (0.95 ± 0.15 mmol/l and 1.31 ± 0.3 mmol/l, respectively) (p<0.05). The islet morphology as examined by immunocytochemistry using insulin antibodies in the L-STZ/2HF group was affected and quantitative analysis showed the islet insulin content was higher than that of rats with type 1 diabetes (P<0.05). We concluded that the new rat model of type 2 diabetes established with conjunctive treatment of low dose of STZ and high fat diet was characterized by hyperglycemia and light impaired insulin secretion function accompanied by insulin resistance, which resembles the clinical manifestation of type 2 diabetes. Such a model, easily attainable and inexpensive, would help further elucidation of the underlying mechanisms of diabetes and its complications.
  • Hiroyuki NAITO, Amane NAKAMURA, Maki INOUE, Yoshihiko SUZUKI
    原稿種別: Original
    2003 年 52 巻 5 号 p. 409-414
    発行日: 2003年
    公開日: 2003/10/22
    ジャーナル フリー
    Ultrasonic vocalization (USV) responses elicited by air-puff stimuli were compared in regard to both quality and quantity with those elicited by electric foot-shock(s) in adult rats. Frequency pattern, duration, repetition rate and interpulse interval of air-puff-elicited USV were comparable to those observed on foot-shock-elicited USV. Diazepam (0.25-1.0 mg/kg, s.c.) and buspirone (0.1-1.0 mg/kg, s.c.) attenuated equally and dose-dependently the USV responses elicited by both aversive stimuli. Air-puff-elicited USV was specifically attenuated in a dose-dependent manner by the anxiolytic properties of several psychotropic agents: diazepam (1.0-10.0 mg/kg, p.o.), buspirone (10.0-100.0 mg/kg, p.o.), 8-OH-DPAT (0.01-0.5 mg/kg, s.c.). Haloperidol (0.2-1.0 mg/kg, s.c.) weakly attenuated the USV response. Imipramine (0.2-1.0 mg/kg, s.c.) which has no anxiolytic property had no effect. Consequently, air-puff-elicited USV as well as foot-shock-elicited USV may provide a reliable tool for the study of anxiety.
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