Journal of Equine Science
Online ISSN : 1347-7501
Print ISSN : 1340-3516
ISSN-L : 1340-3516
Volume 22 , Issue 1
Showing 1-3 articles out of 3 articles from the selected issue
ORIGINAL ARTICLE
  • Masafumi HASHIMOTO, Yasuo NAMBO, Takashi KONDO, Kiyotaka WATANABE, Koi ...
    Type: -Original-
    2011 Volume 22 Issue 1 Pages 1-7
    Published: 2011
    Released: April 26, 2011
    JOURNALS FREE ACCESS
    In mammal circulation, ferritin-binding proteins (FBPs) are thought to be involved in clearance of circulating ferritin after complex formation with it through receptor-mediated uptake. However, there is no report on fetal FBP in fetal circulation. Although iron concentrations of fetal horse plasma were higher than those of adult horse plasma, plasma ferritin concentrations and ferritin-binding activities were found to be significantly lower in fetus than in adult. FBPs were purified from fetal or adult horse plasma on horse spleen ferritin-Sepharose 4B affinity column. Partially affinity-purified fetal horse plasma FBPs were mainly separated into 65 and 41 kDa bands in addition to minor bands with higher molecular masses ranged from 102 to 140 kDa on SDS-PAGE under reducing condition. The adult horse plasma FBPs were separated into 74, 54 and 28 kDa bands, and the 74 and 54 kDa bands reacted with antibodies specific for horse IgM and IgG heavy chains, respectively, by immunoblotting analyses. On the other hand, no antibodies to horse immunoglobulin classes detected any bands in fetal horse plasma FBPs. The affinity-purified adult and fetal horse plasma FBPs did not contain fibrinogen as a plasma specific FBP, probably due to its lower affinity to the ligand ferritin. These results demonstrate the presence of FBPs which are different from adult horse plasma FBPs including anti-ferritin autoantibodies in fetal plasma.
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  • Pramod DHAKAL, Nobuo TSUNODA, Rie NAKAI, Kentaro NAGAOKA, Yasuo NAMBO, ...
    Type: -Original-
    2011 Volume 22 Issue 1 Pages 9-15
    Published: 2011
    Released: April 26, 2011
    JOURNALS FREE ACCESS
    The aim of present study was to clarify the post-natal profile of follicle-stimulating hormone (FSH), luteinizing hormone (LH), immunoreactive (ir)-inhibin, progesterone, testosterone, and estradiol-17β, and their relationships in Thoroughbred colts. Six hundred and thirty-six colts were used for the study. Single plasma samples from each animal were harvested from the blood drawn through jugular venipuncture. The subjects were born with high amounts of progesterone, testosterone, and estradiol-17β, all of which dropped significantly and remained at lower levels till the end of 6 months. FSH decreased transiently after birth until day 12 and then gradually increased to peak at day 100 which then maintained in lesser levels towards the end of the studied period. LH was highest during birth which decreased until day 26 and then increased slowly to sub-birth levels up to day 90. Animals were born with high amounts of ir-inhibin. It dropped slowly and halved by day 20 and then decreased towards rest of the studied period. The increase in FSH is negatively correlated with the declining ir-inhibin levels. The early increase in FSH can be the indication of early post-natal maturation of the hypothalamic pituitary testicular axis that ultimately might be responsible for priming the testes for future development.
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  • David O. EHIZIBOLO, Amahyel M. GUSI, Peter O. EHIZIBOLO, Elsie U. MBUK ...
    Type: -Original-
    2011 Volume 22 Issue 1 Pages 17-19
    Published: 2011
    Released: April 26, 2011
    JOURNALS FREE ACCESS
    Despite the endemicity of brucellosis in Nigeria, reports on equine brucellosis are rare. The Rose Bengal Plate Test (RBPT) was used to determine the serologic prevalence of Brucella abortus antibodies amongst 75 horses from three stables in two States of northern Nigeria. The highest prevalence (22.7%) was recorded in stable C located in Plateau State and the lowest (6.7%) was found in stable B in Kaduna State. The overall prevalence determined was 14.7%. There is need for the inclusion of horses in brucellosis surveillance and control strategies in Nigeria to safeguard people at high risk.
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