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Volume 13, Issue 2
Displaying 1-7 of 7 articles from this issue
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K. MATSUI, A. L. CARSTEN, E. P. CRONKITE1972 Volume 13 Issue 2 Pages 59-70
Published: June 01, 1972
Released on J-STAGE: August 29, 2006
JOURNAL FREE ACCESSMouse bone marrow cells were stored for 0.5, 16 and 24 hours under one of the following four conditions; saline G at room temperature, saline G at ice temperature, CMRL 1066 at room temperature and CMRL 1066 at ice temperature. Their viability was then examined by counting CFU in the spleen of irradiated recipient mice.
1) The CFU decreased rapidly with storage time under all four conditions. Among four storage conditions, the storage in saline G at ice temperature seemed to give slightly more favorable results than others.
2) Types of micro-colonies were not significantly different under the present storage conditions.
3) Since the size of endogenous colonies were relatively small compared to exogenous colonies, the observed colonies are likely to be resulted from injected bone marrow elements and some endogenous cells.
4) It is concluded that the storage conditions in the present paper are not suitable for the storage of bone marrow cells. This emphasizes the need of exploration of a better storage method.View full abstractDownload PDF (627K) -
O. NIKAIDO, M. HORIKAWA, T. TANAKA, T. SUGAHARA1972 Volume 13 Issue 2 Pages 71-80
Published: June 01, 1972
Released on J-STAGE: August 29, 2006
JOURNAL FREE ACCESSBone marrow cells obtained from both femora of 30-day-old NC-mice were cultured stationarily for varying days in vitro to study the pattern of growth and differentiation of the hemopoietic cells. Within 10 days of culture, erythroid cells, myeloid cells and lymphocyte-like cells disappeared from the culture, while the number of large mononuclear cells increased gradually with cultivation time and constituted about 98% of total cell population at 10 days of culture. On the other hand, these cultured bone marrow cells showed neither protective ability to lethally irradiated mice nor colony forming ability in the spleen of recipient mice, when injected into 700 R-irradiated NC-mice.View full abstractDownload PDF (476K) -
N. YAMAGATA, T. YAMAGATA1972 Volume 13 Issue 2 Pages 81-90
Published: June 01, 1972
Released on J-STAGE: August 29, 2006
JOURNAL FREE ACCESSThyroid glands obtained from the instantaneous victims in Tokyo during the period from September to December 1962, when the fallout rate was the highest, were analyzed for the stable iodine. The average concentration was twice as much as the ICRP data (4×10-4 g/g), namely, 0.85 mg/g fresh tissue, ranging from 0.45 to 2.48. The dietary intakes of iodine by Japanese people are suggested as about 300μg on the average with a basic diet containing a minimum of seaweed and incidental consumption of seaweed may lead to a maximum of 10 mg intake. No significant correlation was found between the levels of 131I and stable iodine in the thyroids in this period, probably due to a wide variation of 131I intake among people. The use of ICRP data for Japanese will give a conservative estimate in the exposure calculation.View full abstractDownload PDF (375K) -
T. MATSUNAMI, A. MIZOHATA, A. FUJITA, T. MAMURO1972 Volume 13 Issue 2 Pages 91-94
Published: June 01, 1972
Released on J-STAGE: August 29, 2006
JOURNAL FREE ACCESSMonthly deposition of 137Cs at Osaka was measured in the period of 7 years from July 1964 through July 1971. Gamma-ray spectra of the samples collected monthly by the basin method were measured with a large volume Ge(Li) detector shielded with 20 cm iron in order to determine their 137Cs activities. On close examination of the variation of monthly 137Cs deposition, it is noted that, besides the seasonal variation of so-called “spring maximum”, there appeared many peaks, each of which can be assumed to have been caused by the influence of a nuclear explosion. Yearly 137Cs depositions during the period were also estimated and compared with those measured by another institute.View full abstractDownload PDF (145K) -
Y. HONDA, Y. KIMURA, Y. TAMURA, C. TANAKA1972 Volume 13 Issue 2 Pages 95-99
Published: June 01, 1972
Released on J-STAGE: August 29, 2006
JOURNAL FREE ACCESSThe experimental studies on uptake and accumulation of 106Ru by artificially fertilized eggs, advanced fry and fingerlings of rainbow trout have been conducted. The accumulation in the egg previously treated with drugs for control of bacterial growth on the egg was more rapid and higher than that in non-treated egg. The major part of 106Ru in the egg was associated with the egg capsule on hatching. The accumulation in the fry increased with growth and the transfer through alimentary tracts with development of digestive organs was observed.View full abstractDownload PDF (180K) -
F. SATO, S. TSUCHIHASHI, W. NAKAMURA, H. ETO1972 Volume 13 Issue 2 Pages 100-108
Published: June 01, 1972
Released on J-STAGE: August 29, 2006
JOURNAL FREE ACCESSAbout 1300 mice were exposed to graded doses of x-rays given by whole or partial body irradiation. For the partial body irradiation, the following three groups were assigned : head exposure, trunk exposure (chest and abdomen) and lower body exposure (pelvis, legs and tail). LD50(30)s were determined for each of the exposures. Daily death distributions after the exposure were observed in 30 days. The partial body irradiations produced specific daily death distributions, specific to the region exposed. The relative patterns of the daily death distributions were nearly independent on the doses over a wide range. Consequently there appeared plateaus in dose versus survival time curves after the head or the lower body exposure. The one after the head exposure could be attributed to oral death. There is no information on a cause of death for the plateau region of the lower body exposed group.View full abstractDownload PDF (316K) -
K. SHINOHARA, S. OKADA1972 Volume 13 Issue 2 Pages 109-116
Published: June 01, 1972
Released on J-STAGE: August 29, 2006
JOURNAL FREE ACCESSMouse lymphocytic leukemia L 5178 Y in colonies showed the decreased radiosensitivity over that in free cell suspension. The decreased radiosensitivity is not sufficiently explained with the increased fraction of stationary phase cells, nor with cell-to-cell interaction, nor with protection by agar in the plating medium. The main cause is likely to be the depletion of oxygen in the multi-cellular system.
The radiosensitivity of stationary phase cells in free cell suspension was slightly, but significantly lower than that of log phase cells.View full abstractDownload PDF (303K)
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