All issues
Volume 23 (1982)
- Issue 4 Pages 411-
- Issue 3 Pages 261-
- Issue 2 Pages 129-
- Issue 1 Pages 88-
Volume 23, Issue 2
Displaying 1-13 of 13 articles from this issue
- |<
- <
- 1
- >
- >|
-
SHOZO SUZUK, OSAMU DOI, YUZURU AKAMATSU1982 Volume 23 Issue 2 Pages 129-140
Published: June 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSThe membrane lipid of murine fibroblast LM cells, which can grow in serum-free medium, was modified by adding an unsaturated fatty acid (palmitoleate, oleate, linoleate or linolenate) to the medium. After irradiation with 60Co γ-rays at 0°C or 25°C, these cells lost their intracellular K+ with time, and the degree of loss varied depending on the modification of their membranes: palmitoleate-supplemented membranes were most resistant to radiation, whereas linolenate-supplemented ones were least resistant at 4°C and 25°C. No membrane constituents other than lipids seemed to be appreciably modified, judging from the indirect evidence that the rates of 42K incorporation into linolenate-supplemented and -unsupplemented cells at 37°C were the same. Thus, these differences in the damage indicate that membrane lipids take part in the radiation-induced change in K+ permeability of LM cells.View full abstractDownload PDF (470K) -
H. AUDA, Z. KHALEF1982 Volume 23 Issue 2 Pages 141-149
Published: June 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSThe kinetics of degradation and synthesis of DNA and the nature of radioactive substances released from M. radiodurans R115 labeled with thymidine-methyl-3H after UV and gamma irradiations were investigated. The release of labeled material from the DNA began immediately upon incubation and terminated in due time 90 min and 180 min for UV and gamma irradiations, respectively. When acriflavine was added to the medium, post-irradiation degradation process did not terminate even after 9 h in the case of UV exposure. However, it terminated after 6 h in the case of gamma irradiation. In the presence of acriflavine, DNA synthesis resumed after termination of DNA degradation in the case of gamma irradiation and this was not observed in the case of UV irradiation.
Degradation products were chromatographed and it was found that they were located in one major radioactive peak. However their locations were different for UV and gamma radiations. For UV irradiation, the peak fell in the thymine region, while for gamma irradiation it fell in the thymidine region.View full abstractDownload PDF (296K) -
ATSUHIKO TAKEDA, NORIO KATOH, MORIO YONEZAWA1982 Volume 23 Issue 2 Pages 150-167
Published: June 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSRadiation protection by post-irradiation injection of a thermostable fraction of the ginseng extract in mice, rats and guinea pigs was studied. The thermostable fraction lost “by-effects” of decrease in body weight and splenic hyperplasia which were caused in injected mice by the original ginseng extract. The fraction protected mice (male) irradiated with 720 R of X-rays and rats (male) irradiated with 825 R with the dose about 6 mg per 100 g of body weight. The fraction also protected guinea pigs, both female and male, irradiated with 325 R with the dose about 80 mg per 300 g of body weight. The thermostable fraction stimulated recovery of thrombocyte and erythrocyte counts, but not leukocyte counts, in 550-R irradiated mice. Recovery of all the three blood cell counts was stimulated by the fraction in rats irradiated with 630 R and guinea pigs irradiated with 200 R. Comparison of stimulated recovery by the thermostable fraction of the ginseng extract among the three blood cell counts showed that restoring action was the most marked on thrombocyte counts, commonly in the three species of the animals.View full abstractDownload PDF (468K) -
SUEMI SUGAVARA, MIKIO SHIKITA1982 Volume 23 Issue 2 Pages 168-175
Published: June 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSAfter whole-body irradiation of mice with 300R X-rays, frequency of myeloid stem cells (CFU-C) in the femoral marrow decreased markedly within 24 hrs and returned to a nearly normal level in 3 or 4 days, while it decreased again thereafter. Bone marrow cells isolated from these mice were plated in semi-solid agar, and exposed against X-rays for the second time. After the cells were cultured for 1 week for colony formation, no significant difference was observed in the survival ratio between the CFU-C of the control and the CFU-C of the animals irradiated 24 hrs before. The result supports the assumption that recovery of the cells occurred quickly during the fractionated irradiation. In contrast, a slightly increased radiosensitivity of CFU-C was observed 3 or 4 days after the initial irradiation, while it was no longer observed 7 days after irradiation. It seems that latent injury was expressed through amplification during repeated cell divisions, and that a mechanism operated rather slowly for the recovery of this kind of injury.View full abstractDownload PDF (353K) -
A. RAMESHA RAO, P.N. SRIVASTAVA1982 Volume 23 Issue 2 Pages 176-186
Published: June 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSPresent communication reports the quantitative changes in the oocyte population of adult Indian desert gerbil (Meriones hurrianae) exposed to internally deposited 32P, 60Co and 45Ca. The nuclides were administered intraperitonially at the dose levels, in each case, of 5.55 kBq, 11.1 kBq, 18.5 kBq, 37 kBq and 74 kBq per gram body weight and the animals were sacrificed at the intervals of 1, 3, 7, 15 and 30 days. The depletion of oocyte population was dependent, in case of each nuclide, upon the dose administered as well as the time lapsed after the treatment. 32P and 60Co were more effective in damaging the oocytes than was 45Ca. However, histological sterility was not produced by any of these nuclides.View full abstractDownload PDF (363K) -
JUNJI MIYAKOSHI, SHIN-ICHIRO HEKI, MASAYO FURUKAWA, EIICHI KANO1982 Volume 23 Issue 2 Pages 187-197
Published: June 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSThe recovery kinetics from the synergistic damage resulting from the combination treatments of X-irradiation (3 Gy), and 42°C (60 min) and 44°C (15 min) hyperthermia were analized in Chinese hamster V-79 cells. Recovery time was less than about 8 hr when the first treatment was hyperthermia [42°C (60 min) or 44°C (15 min)] alone, X-irradiation alone, the combined treatments with the both, or the combined hyperthermia of 42 → 44°C sequence. However, when the combined hyperthermia of 44 → 42°C sequence was given as the first treatment, the survival of cells did not attain to the additive level within a 24 hr interval at 37°C. From these results, it is suggested that the damage induced by the combined hyperthermia of 44 → 42°C sequence was retained partially in the cells during at least a 24 hr interval at 37°C, and may interact synergistically with the damage by the subsequent X-irradiation.View full abstractDownload PDF (443K) -
YUKIO TAKIZAWA1982 Volume 23 Issue 2 Pages 198-203
Published: June 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSThe accumulation of 239+240Pu in human tissues in Japan for the last 15 years, since 1962, was determined in order to correlate with possible fluctuations in environmental pollution. The results showed that the cumulative amounts of 239+240Pu found in gonads and bone exhibited a high activity among the several organs analyzed at this point. It is apparent that 239+240Pu has an affinity for these tissues. The relatively high levels of 239+240Pu in the gonads and in the bones as determined by this research warrants additional attention for its possible dangers and, hence, for the necessity of determining the implication of these hazards.View full abstractDownload PDF (222K) -
K. KUBO, S. KOIWAI, K. MORITA1982 Volume 23 Issue 2 Pages 204-217
Published: June 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSThe Radioresistant strain (X-8-5) was obtained from HeLa-SC population X-irradiatedd repeatedly for five times with 800 rad. The mean lethal dose (D0) was 196 rad for X-8-5 cells, while it was 166 rad for control HeLa-SC cells. The fraction of cells containing an unusually long acrocentric chromosome (LA 2) exclusively increased with increasing number of irradiation of HeLa-SC population. A clonal strain with LA 2 marker was isolated from X-8-5 population and named RC-355. Since the RC-355 cells were more resistant (D0=220 rad) than parental X-8-5 cells (D0=196 rad), it was suggested that the cells with LA 2 were responsible for the radioresistance of X-8-5 population. The RC-355 cells were further subjected to the analysis of Q-banded karyotypes and it was observed that 18 types of specific markers (rm 1-17 and LA 2) were included in RC-355 cells in addition to 12 types of markers observed in most of HeLa-SC cells. Since the analysis of Q-banded karyotypes of RC-355 cells showed that RC-355 specific markers were not produced by radiation-induced rearrangements of HeLa-SC chromosomes, because twelve kinds of HeLa-SC markers were presented in RC-355 cells without any change, it was concluded that a small number of cells with LA 2 marker were originally presented in the control population and the relative fraction of them occupied increased after irradiation.View full abstractDownload PDF (473K) -
EIICHI KANO, JUNJI MIYAKOSHI, MASAYO FURUKAWA, HIROKAZU KATO, SUSUMU O ...1982 Volume 23 Issue 2 Pages 218-227
Published: June 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSV-79 cells were X-irradiated and/or heated at 42°C, 44°C and 50°C in water bath and colonized in vitro. To and Tq at 50°C were estimated as about 0.1 minute and theoretically less than 1.0 minute respectively. Arrhenius like plot did''nt bend at any temperatures between 43°C and 50°C, as reported elsewhere1). Preheated cells at 42°C for 2 hours were resistant to the successive heating at 50°C, while those at 50°C for 80 seconds were sensitized to that at 42°C. The survival fraction ratio, i.e. products of survival fractions from independent treatment with 4.5 Gy and with 50°C, respectively, divided by a survival fraction from a combined treatment with 4.5 Gy and 50°C, showed that the longer 50°C heating period gave the more synergistic damage. Postirradiation heating periods were conditioned to give the equal survivals from single heat treatment at 42°C, 44°C and 50°C for the estimation of thermal enhancement ratio in Do, i.e. 1.98, 1.98 and 1.64 respectively. Heating at 50°C showed the given cell killing rate for the shortest period among the temperatures assayed but did''nt seem essentially to give the more thermal radio-enhancement ratio by itself.View full abstractDownload PDF (380K) -
HIROKAZU KATO, TETSUYA ISHIDA1982 Volume 23 Issue 2 Pages 228-233
Published: June 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSA non-invasive applicator for local deep hyperthermia was deviced. The applicator is composed of a one-turn square coil in columnar shape through which the RF current flows. The material to be heated is placed at one of the external sides of the coil.
The energy deposited at a depth of 11 cm by the RF applicator, 20 cm in height, 20 cm in width and 60 cm in length, was estimated 17% of that at the depth of 1 cm. The assessment was made on the condition that the two applicators be placed face to face at a distance of 22 cm. The energy deposited at a depth of 11 cm was estimated 68% of that at the depth of 1cm.View full abstractDownload PDF (160K) -
YOSHIHARU KARUBE, TATSUO MAEDA, MASATO OHYA, SETSURO SUGATA, AKIRA KON ...1982 Volume 23 Issue 2 Pages 234-241
Published: June 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSSixteen 99mTc labeled ligands were evaluated as scintigraphic agents. The ligands studied were cysteine, glutathione, their S-substituted derivatives, lysine-N∈ N∈-diacetic acid, glycylglycine-N, N-diacetic acid, glycylglycylglycine-N, N-diacetic acid, taurine-N, N-diacetic acid, hydrazine-N, N-diacetic acid, ethylenediamine-N, N-diacetic acid, and propylene-l, 3-diamine-N1-, N1-diacetic acid. The ligands were labeled with 99mTc by the SnCl2 method with more than 95% yield. The in vivo behavior of the 99mTc labeled ligands were studied in golden hamsters and dogs. The organ distribution in golden hamsters indicated clearance both by hepatobiliary and renal systems. The pancreas/blood ratios were much lower in the 99mTc ligands than in 75Se-selenomethionine. Scintigraphic studies in dogs showed that the liver and kidneys were well visualized but the accumulation by the pancreas was not sufficient for clear visualization.View full abstractDownload PDF (364K) -
H. OKABAYASHI1982 Volume 23 Issue 2 Pages 242-252
Published: June 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSThe amount of Po-210 excreted in urine and feces was more influenced by Po-210 that was taken with food and drink than taken through inhalation.
The amount of Pb-210 in urine of mining workers among uranium mine workers was higher than that of the non-uranium mine workers. It was thought that this fact was due to the working environment in uranium mine the amount of Pb-210 being a few tens times higher than that in normal environment.
The activity ratios of Po-210 of faecal to urinary excretion are widely distributed, however, the average value of many samples approached to 10.
Urinary excretion of Po-210 was highest after 24 hours of ingestion, but for faecal excretion, it was highest after 3 days.View full abstractDownload PDF (378K) -
MOHAMMAD MOMDOOH HELAL GHONEUM, KEN-ICHI IJIRI, NOBUO EGAMI1982 Volume 23 Issue 2 Pages 253-259
Published: June 15, 1982
Released on J-STAGE: June 16, 2006
JOURNAL FREE ACCESSThe effects of gamma-rays on the thymus in the adult fish of Oryzias latipes were studied. Fish were subjected to a single whole-body irradiation of the dose ranging from 0 to 8 kR. Through the histological sections, the quantitative treatment was performed on the changes in thymus volume and mitotic activity of thymocytes after irradiation. The thymus in the irradiated fish exhibited a sudden drop in its volume on day 1-3 post-irradiation, subsequently followed by its regeneration. The change in mitotic index of thymocytes, especially in the regeneration phase, showed a dose-dependent behavior (same level as the control for 10 and 25 R; an overshoot for 100 and 200 R; a gradual recovery for 1 and 2 kR; no mitosis for 4 and 8 kR).View full abstractDownload PDF (290K)
- |<
- <
- 1
- >
- >|