This report describes four cases of oral cancer treated with immunotherapy using lymphokine activated killer (LAK) cells and radiotherapy. In this study, the cytotoxicity and surface phenotype of induced LAK cells were analyzed. Peripheral blood lymphocytes (PBLs) were also analyzed before and after LAK cell infusion into oral cancer patients. Furthermore, the change of the affected part of the tumor using immunohistochemical procedure was observed.
PBLs were obtained from oral cancer patients and induced to LAK cells by rIL-2 (Shionogi Pharmaceutical Co, Ltd, 700JRU/ml) and anti-CD3 moAb (25ng/ml) by culturing for 17 to 24 days. Infusions into regional artery of the tumor in patients were 8-fold divided LAK cells obtained by culturing twice, each infusion interval was 2 to 3 days, and 6.2-17×10⁹ LAK cells were infused into each patient. The cytotoxicity was determined in a 4-hour ⁵¹Cr release assay and was determined by K-562 cells (E/T=5/1) . Phenotypic characterization was determined by dichromatic flow cytometry using several monoclonal antibodied. The determined cells were LAK cells and PBLs before and after infusion of LAK cells into oral cancer patients. As for results, cytotoxicity of the mean of LAK cells was 74.6%. Cytotoxicity of PBLs between before and after infusion of LAK cells showed the tendency to decrease in in vitro determination. As for phenotypic characterization, CD8⁺/CD11^- cells and CD3⁺/HLA-DR⁺ cells were increased with the mean of LAK cells. CD4⁺ cells were increased, CD8⁺/CD11^-cells, CD3⁺/HLA-DR⁺ cells, and CD8⁺/CD11⁺ were decreased with the mean of PBLs between before and after infusion of LAK cells. After infusion of LAK cells, infiltration of UCHL-1⁺ cells and HLA-DR⁺ cells were increased markedly around the tumor in case 1.

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A case of adenosquamous carcinoma arising in the maxillary sinus is reported with keratin immunohistochemical evaluations.
Adenosquamous carcinoma is a very rare carcinoma of the maxillary sinus. Three cases of adenosquamous carcinoma arising in the maxillary sinus have been reported since 1966. Histopathologically, tumor tissue composes of tubular carcinoma and squamous cell carcinoma cells. Tissue specimens were evaluated with monoclonal antibody (K4, K7, K8, K10, K13, K17, K18, K19, K20, KL1, and K8.12) using the ABC method. Tubular carcinoma cells were positive with K7, K8, K18, K19, KL1, and K8.12 and squamous cell carcinoma cells were strongly positive with K7, K10, KL1, and K8.12 and weakly positive with K4 and K18. Tubular carcinoma cells formed keratin pairs of K8-18, and squamous cell carcinoma cells formed keratin pairs of K7-17.

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Exfoliative cytology is useful for early diagnosis of malignant or suspected lesions, how-ever, our previous study showed that oral cytology for T1 or leukoplakic lesions is not as reliable as that for T2-4 or exo- or endophytic lesions. An interdental brush was used for sample collection to improve the correct cytodiagnosis rate. Fifty-one smears obtained from the patients with T1 and T2 squamous cell carcinoma of the oral cavity were evaluated clinicocytologically. By using an interdental brush, the percentage of correct cytodiagnosis of T1 increased from 76.1% to 94.4%; in particular, the percentage of Class TV/V significantly increased from 43.5% to 77.8%. Exfoliative cytology using an interdental brush is a simple and reliable technique that aids early diagnosis of oral and oropharyngeal malignancies, particularly T1 and T2 lesions that can be treated with excisional biopsy.

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A suspected case of radiation-induced cancer 13 years after initial treatment for tongue cancer was reported. The patient was a 61-year-old female, treated by 60Gy radiotherapy for tongue cancer of the right side. Seven years later, recurrent tongue tumor arose and was irradiated up to the total dosage of 82.5Gy.
Thirteen years after the initial treatment, a tumor arose near the flap resembling a mucocele at first, and its growth was rapid. She underwent surgery in 1993. Histopathological examination revealed partial mesenchymal features, but it was difficult to determine its origin. Based on Sakai's criteria, the points of location, latency, and histopathological observation, the tumor was considered to be radiation-induced cancer.

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Von Recklinghausen's disease or Neurofibromatosis (NF) is an autosomal dominant disorder with a frequency of 1 in 3000. Characteristically, the symptoms of patients are cafe-aulait spots, neurofibromas of the peripheral and central nerve systems, and skeletal abnormalities which develop during infancy and childhood. NF is known to be complicated by malignant tumors, which are mainly of soft tissue origin. Among head and neck malignancies, the number of soft tissue sarcomas is the most limited and this type of tumor in combination with NF is rarest. In this report, a rare case of malignant schwannoma of the mandible in von Recklinghausen's disease is presented. The patient was a 7-year-old female who complained about swelling of the right mandibular region and disturbance of mouth opening and closing. Clinically, the lesion was an elastic, hard mass about 5-cm in diameter at the right mandibular ramus. Biopsy was performed, and the histological diagnosis was malignant schwannoma. Though surgical excision, intraoperative irradiation and adjuvant chemotherapy were carried out, local recurrence and lung metastasis occurred and she died 1 year 4 months after initial diagnosis.

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