The objectives of research were to characterize the effects of prostaglandins (PG) on the release of growth hormone (GH), prolactin (PRL), and luteinizing hormone (LH) in cultured bovine anterior pituitary cells. PGE
2 significantly increased the media concentrations of GH by 141% (P<0.01) and 27% (P<0.01) at the concentrations of 10
-7 and 10
-8 M, respectively, compared with controls. The PGE
2 significantly increased media concentrations of PRL compared with controls at the concentrations of 10
-7 and 10
-8 M by 120% (P<0.01) and 22% (P<0.05), respectively. PGE
2 also significantly increased LH release at the concentration of 10
-7 M by 22% (P<0.05), but not at a concentration of 10
-8 M. PGF
2α significantly increased the release of LH compared with controls at the concentrations of 10
-8 and 10
-9 M by 15% (P<0.05) and 19% (P<0.05), respectively, while PGF
2α failed to stimulate the release of GH and PRL. In contrast to PGE
2 and PGF
2α, PGD
2 had no effect on the release of GH, PRL, and LH. These results suggest that PGE
2 is the most potent prostaglandin among the PGs for the release of GH, PRL, and LH by acting directly on bovine pituitary cells.
View full abstract