Two experiments were conducted to compare the effect of estrus induction by controlled internal drug release (CIDR) and intravaginal cream containing 500 mg progesterone (P cream) in ewes during the non-breeding season. In the first experiment, twenty-four ewes were randomly grouped for two treatments with the different intravaginal devices for 12 days: Group A was the CIDR group and Group B was the P cream group. Blood was collected from all treated ewes, and progesterone (P
4), estradiol 17-
β (E
2) and luteinizing hormone (LH) concentrations were measured by enzyme immunoassay. In the second experiment, the conception rates from natural mating, estrus-detected AI (inseminated 12 h after estrus detection), or fixed-time AI (inseminated 42 h after removal of an intravaginal device) in 127 ewes treated with CIDR or P cream were compared. In Experiment 1, the rate of estrus induction and the time of estrus onset after device removal were 91.7% and 36.3 ± 15.7 h in Group A, and 100% and 35.0 ± 12.6 h in Group B, respectively. There were no significant differences between the devices. The mean plasma P
4 concentration in Group B was significantly (P<0.01) lower than Group A between day -9 and day -1 (Day 0: the day of device removal). However, no significant differences were found in the mean E
2 concentrations of the two groups after treatment. The mean time of estrus onset in ewes with an observed LH surge and the time of LH surge after treatment were 23.3 ± 8.7 h and 30.3 ± 5.0 h for Group A and 27.6 ± 6.5 and 26.3 ± 8.0 h for Group B, respectively, and there were no significant differences. However, a significant difference (P<0.05) was found in the mean time from the time of estrus onset to LH surge between Group A (6.4 ± 6.7 h) and Group B (-1.3 ± 4.1 h). In Experiment 2, the conception rates for natural mating, estrus-detected AI, and fixed-time AI were 55.0, 29.4, and 25.0% for Group A and 40.7, 25.0, and 42.1% for Group B, respectively, and there were no significant differences. These results suggest that the effect of induction of estrus and ovulation and the rate of conception after treatment were comparable to CIDR even though the plasma P
4 concentration of the P cream method tended to be low during the insertion period.
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