Previous reports suggested the involvement of alpha-tubulin N-acetyltransferase 1 (ATAT1) in flagella formation in spermatozoa; however, whether ATAT1 is expressed in flagella and involved in spermatozoa maturation and capacitation remains to be elucidated. Yanai et al. evaluated the expression of ATAT1 in the male reproductive system in mice using immunostaining and western blotting (Yanai et al. Expression and localization of alpha-tubulin N-acetyltransferase 1 in the reproductive system of male mice. pp. 59–66). The localization of ATAT1 protein in the male germline was detected during spermiogenesis as well as spermatozoa maturation. Therefore, these results suggest that ATAT1 might be involved not only in flagella formation, but also in the acetylation process during spermatozoa maturation and capacitation.