A rapid and sensitive determination of acrinol in pharmaceuticals was investigated by spectrofluorometric flow injection analysis. The single flow system was assembled to depress dispersion of the sample solution. The sample was dissolved in the buffer solution (pH 4) and acetone to give 30 v/v% acetone solution and 100 μl of sample solution was injected into the buffer stream. The mixing coil was 1 m in length. The fluorescence intensity of acrinol was measured at excitation and emission wavelengths, 366 and 496 nm. The intensity was enhanced by adding polar solvents such as methanol, ethanol, 1-propanol and acetone. Of these solvents, the 30 v/v% acetone solution was most effective. The calibration graph showed a good linear relationship between 1×10
-9 M7×10
-8M of acrinol and the dynamic range was wide. The sensitivity was enhanced about 1000 times by the colorimetry compared with other methods. The lower detection limit was 7.5×10
-10M (
S/
N=3). The relative standard deviation was 0.5% for 10 repeated runs of 5×10
-8 M acrinol and a sampling rate of 60/h. Many substances such as berberine and dibucaine showed fluorescence, but had different excitation and emission wavelengths. Accordingly, these substances did not interfere with the determination of acrinol. The proposed method can be successfully applied to the rapid, accurate and trace determination of acrinol in pharmaceuticals with a diversified matrix.
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